論文 - 詳細
| RRC ID | 33458 |
|---|---|
| 著者 | Löwenadler B, Jansson B, Paleus S, Holmgren E, Nilsson B, Moks T, Palm G, Josephson S, Philipson L, Uhlén M. |
| タイトル | A gene fusion system for generating antibodies against short peptides. |
| ジャーナル | Gene |
| Abstract |
A novel method to obtain specific antibodies against short peptides is described, involving synthesis of the corresponding oligodeoxynucleotides followed by cloning into a new set of fusion vectors, pEZZ8 and pEZZ18, based on two synthetic IgG-binding domains (ZZ) of Staphylococcus aureus protein A. The soluble gene fusion product thus obtained, can be collected from the culture medium of Escherichia coli and rapidly recovered in a one-step procedure by IgG affinity chromatography. The system was used to express a fusion protein consisting of the two Z fragments and the C-terminal part [amino acids (aa) 57-70] of human insulin-like growth factor I (IGF-I). This 16-kDa protein was purified by affinity chromatography on IgG Sepharose and antibodies were raised in rabbits. The fusion protein elicited peptide-specific antibodies, as measured by solid-phase radioimmuno assay and Western blotting, reactive with both synthetic C-terminal peptide and the native human IGF-I protein. The results suggests that the gene fusion system can be used for efficient antibody production against short peptides encoded by synthetic oligodeoxynucleotides. |
| 巻・号 | 58(1) |
| ページ | 87-97 |
| 公開日 | 1987-1-1 |
| DOI | 10.1016/0378-1119(87)90032-1 |
| PII | 0378-1119(87)90032-1 |
| PMID | 3692175 |
| MeSH | Amino Acid Sequence Antibody Formation* Base Sequence Cloning, Molecular / methods* Genetic Vectors Humans Insulin-Like Growth Factor I / genetics Insulin-Like Growth Factor I / immunology Molecular Sequence Data Oligodeoxyribonucleotides / genetics Peptides / genetics* Peptides / immunology Recombinant Fusion Proteins / genetics* Recombinant Fusion Proteins / immunology Recombinant Proteins / genetics* |
| IF | 2.984 |
| 引用数 | 113 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 15 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | pEZZ18 pEZZ8 |