論文 - 詳細
| RRC ID | 33462 |
|---|---|
| 著者 | Soberon X, Covarrubias L, Bolivar F. |
| タイトル | Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325. |
| ジャーナル | Gene |
| Abstract |
In vitro recombinant DNA experiments involving restriction endonuclease fragments derived from the plasmids pBR322 and pBR325 resulted in the construction of two new cloning vehicles. One of these plasmids, designated pBR327, was obtained after an EcoRII partial digestion of pBR322. The plasmid pBR327 confers resistance to tetracycline and ampicillin, contains 3273 base pairs (bp) and therefore is 1089 bp smaller than pBR322. The other newly constructed vector, which has been designated pBR328, confers resistance to chloramphenicol as well as the two former antibiotics. This plasmid contains unique HindIII, BamHI and SalI sites in the tetracycline resistance gene, unique PvuI and PstI sites in the ampicillin resistance gene and unique EcoRI, PvuII and BalI sites in the chloramphenicol resistance gene. The pBR328 plasmid contains approx. 4900 bp. |
| 巻・号 | 9(3-4) |
| ページ | 287-305 |
| 公開日 | 1980-5-1 |
| DOI | 10.1016/0378-1119(90)90328-o |
| PII | 0378-1119(90)90328-O |
| PMID | 6248430 |
| MeSH | Ampicillin / pharmacology Chloramphenicol / pharmacology DNA, Bacterial / genetics Escherichia coli / genetics* Genetic Vectors* Phenotype Plasmids* R Factors Recombination, Genetic* Tetracycline / pharmacology |
| IF | 2.984 |
| 引用数 | 616 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 43 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | pBR328 |