論文 - 詳細
| RRC ID | 33463 |
|---|---|
| 著者 | Parke D. |
| タイトル | Construction of mobilizable vectors derived from plasmids RP4, pUC18 and pUC19. |
| ジャーナル | Gene |
| Abstract |
Mobilizable narrow-host-range plasmids were constructed from pUC18 and pUC19 by addition of a segment of pSUP2021 bearing the basis of mobilization (bom) site and origin of transfer (oriT) of RP4. One pair of expression vectors, pARO180 and pARO190, retains the beta-lactamase (bla) gene and twelve of the 13 restriction enzyme multiple cloning sites (MCS) of pUC18/19. Another pair was created by replacing the bla gene with the gene encoding kanamycin resistance (kan) from Tn5. The molecules replicate to high copy number in Escherichia coli and Enterobacter aerogenes. They can be transferred efficiently to other Gram- bacteria from the mobilizing strain, E. coli S17-1. In non-enteric strains, the new plasmids can be used as suicide vectors in site-specific insertional mutagenesis. |
| 巻・号 | 93(1) |
| ページ | 135-7 |
| 公開日 | 1990-9-1 |
| DOI | 10.1016/0378-1119(90)90147-j |
| PII | 0378-1119(90)90147-J |
| PMID | 2227423 |
| MeSH | DNA Mutational Analysis Enterobacter / genetics* Escherichia coli / genetics* Genetic Engineering / methods Genetic Vectors* Plasmids Restriction Mapping |
| IF | 2.984 |
| 引用数 | 61 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 14 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | pARO180 pARO181 pARO190 pARO191 |