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  • Search Condition : Filter (MeSH = Genetic Engineering / methods)
Species Resource Title
DNA material pRS406redm-pphlO6-TBG (RDB21033) Designing strong inducible synthetic promoters in yeasts.
Arabidopsis / Cultured plant cells, genes rpc00008 Direct protein delivery into intact Arabidopsis cells for genome engineering.
Cord blood stem cells for research Humanization of Murine Neutralizing Antibodies against Human Herpesvirus 6B.
Human and Animal Cells 253G1(HPS0002) Controlling gene activation by enhancers through a drug-inducible topological insulator.
DNA material , Human and Animal Cells pCriMGET_v1-Tbx3-3xFlag-2A-EGFP (RDB19321) , pCriMGET_v1-Clec4f-IRES-hDTR-2A-EGFP (RDB19322) , pX330-AAVS1-sgRNA (RDB19323) , pCriMGET_v1 (RDB19318) , pX330-syn-crRNA-TS-sgRNA (RDB19319) , pCriMGET_v1-AAVS1-SA-Neo-pA (RDB19320) , 293T(RCB2202) , HeLa Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence.
General Microbes JCM20779 Direct cloning and heterologous expression of natural product biosynthetic gene clusters by transformation-associated recombination.
Human and Animal Cells A549(RCB0098) , 293T(RCB2202) Targeted DNA demethylation in vivo using dCas9-peptide repeat and scFv-TET1 catalytic domain fusions.
Human and Animal Cells 201B7(HPS0001) Generation of an ICF syndrome model by efficient genome editing of human induced pluripotent stem cells using the CRISPR system.
Zebrafish Tg(huC:GVP) , UAS:GCaMP7a Optogenetic control of endogenous Ca(2+) channels in vivo.
Silkworms CRISPR/Cas9-mediated knockout of factors in non-homologous end joining pathway enhances gene targeting in silkworm cells.
Medaka resource database The genomic and genetic toolbox of the teleost medaka (Oryzias latipes).
Prokaryotes E. coli Keio collection Metabolic engineering of Escherichia coli for the production of 1,2-propanediol from glycerol.
DNA material pCAG-EGxxFP (RDB13532) , pX330-Cetn1/sgRNA#1 (RDB13533) , pX330-Prm1/sgRNA#1 (RDB13534). Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA.
Prokaryotes E. coli M13mp10 , M13mp11 New M13 vectors for cloning.
Prokaryotes E. coli pARO180 , pARO181 , pARO190 , pARO191 Construction of mobilizable vectors derived from plasmids RP4, pUC18 and pUC19.
Prokaryotes E. coli pCB182 , pCB192 , pCB264 , pCB267 Promoter-probe vectors for the analysis of divergently arranged promoters.
C.intestinalis / (O.japonicus) Wild C. int Tissue-specific and ubiquitous gene knockouts by TALEN electroporation provide new approaches to investigating gene function in Ciona.
General Microbes JCM 9575 Development of an autonomously replicating linear vector of the yeast Cryptococcus humicola by using telomere-like sequence repeats.
DNA material , General Microbes pxCANCre (RDB1675) , pULwL (RDB1677) , JCM 2499 , JCM 4626 , JCM 4710 , JCM 6230 Genome-minimized Streptomyces host for the heterologous expression of secondary metabolism.
Zebrafish Transient and stable transgenesis using tol2 transposon vectors.