論文 - 詳細
| RRC ID | 33478 |
|---|---|
| 著者 | Patwardhan S, Gupta KC. |
| タイトル | Translation initiation potential of the 5' proximal AUGs of the polycistronic P/C mRNA of Sendai virus. A multipurpose vector for site-specific mutagenesis. |
| ジャーナル | J Biol Chem |
| Abstract |
To determine the translation initiation potential of the 5' proximal four AUGs of the polycistronic P/C mRNA of Sendai virus, we have developed an efficient system for oligonucleotide-directed site-specific mutagenesis utilizing a plasmid vector which contains the replication origin of the single-stranded DNA phage f1 and the phage SP6 promoter. Utilizing uridine containing single-stranded DNA templates of the plasmid vector carrying the P/C gene we introduced point mutations in all four of the putative initiator codons (AUGs) of the P/C gene. Based on the analysis of in vitro translation products from the SP6 polymerase-directed transcripts of the mutants, the first and second AUG codons were assigned to the P and C proteins, respectively. Proteins detected in vitro, Y1 and Y2, initiated from the third and fourth AUG codons, respectively, and originated within the C reading frame. Since the C' protein is encoded in the same reading frame as the C protein but did not initiate from any of the 5' proximal AUGs, the synthesis of the C' protein may start from a non-AUG initiator codon in the P/C gene. |
| 巻・号 | 263(10) |
| ページ | 4907-13 |
| 公開日 | 1988-4-5 |
| PII | S0021-9258(18)68872-6 |
| PMID | 2832411 |
| MeSH | Base Sequence Cloning, Molecular Codon Genes* Genes, Viral* Genetic Vectors Molecular Sequence Data Parainfluenza Virus 1, Human / genetics* Peptide Chain Initiation, Translational* Promoter Regions, Genetic RNA, Messenger / genetics* |
| IF | 4.238 |
| 引用数 | 34 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 原核生物(大腸菌) | pG1f1- pG1f1+ |