Reference - Detail
| RRC ID | 33482 |
|---|---|
| Author | Armstrong KA, Acosta R, Ledner E, Machida Y, Pancotto M, McCormick M, Ohtsubo H, Ohtsubo E. |
| Title | A 37 X 10(3) molecular weight plasmid-encoded protein is required for replication and copy number control in the plasmid pSC101 and its temperature-sensitive derivative pHS1. |
| Journal | J Mol Biol |
| Abstract |
Nucleotide sequences were determined for a region essential for autonomous replication and partitioning of pSC101, a plasmid whose replication is dependent on the Escherichia coli dnaA gene product. The essential replication region contains one long coding sequence, rep101 , for a protein composed of 316 amino acids, and a polypeptide approximately 37 X 10(3) Mr in size was identified as the rep101 gene product. rep101 is preceded by two inverted repeat sequences, three directly repeated sequences and a region of high A + T content containing a sequence similar to the E. coli oriC consensus sequence. Because the lesions in seven replication-deficient insertion mutants, four mutants with increased copy number and one temperature-sensitive replication mutant occur within rep101 , the rep101 gene product must control pSC101 replication and copy number. par, a region adjacent to the replication region, which functions in stable plasmid inheritance, contains several inverted repeat sequences. |
| Volume | 175(3) |
| Pages | 331-48 |
| Published | 1984-5-25 |
| DOI | 10.1016/0022-2836(84)90352-8 |
| PII | 0022-2836(84)90352-8 |
| PMID | 6327996 |
| MeSH | Autoradiography Bacterial Proteins* Base Sequence Chromosome Mapping DNA Replication* Escherichia coli / genetics* Molecular Weight Mutation Plasmids* Repetitive Sequences, Nucleic Acid Temperature |
| IF | 4.76 |
| Times Cited | 118 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | pEL3 |