RRC ID 33483
著者 Hartley RW.
タイトル Barnase and barstar. Expression of its cloned inhibitor permits expression of a cloned ribonuclease.
ジャーナル J Mol Biol
Abstract Barnase is the extracellular ribonuclease of Bacillus amyloliquefaciens and barstar its specific intracellular inhibitor. The gene for barstar has now been cloned and sequenced. When the wild-type gene for barnase is reconstructed from its previously cloned parts on the same plasmid as the barstar gene, the lethal effect of its expression is suppressed. A plasmid has been devised which directs the secretion of 100 mg per active barnase liter by Escherichia coli and another which provides large (500 to 1000 mg/l) yields of barstar. The structure of these plasmids and the derived 89 amino acid sequence of barstar are reported.
巻・号 202(4)
ページ 913-5
公開日 1988-8-20
DOI 10.1016/0022-2836(88)90568-2
PII 0022-2836(88)90568-2
PMID 3050134
MeSH Amino Acid Sequence Bacillus / genetics Bacterial Proteins / genetics* Base Sequence Cloning, Molecular* DNA, Bacterial Escherichia coli / genetics Genes, Bacterial Molecular Sequence Data Plasmids Ribonucleases / antagonists & inhibitors Ribonucleases / genetics*
IF 4.76
引用数 224
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌) pMT416