論文 - 詳細
RRC ID | 33483 |
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著者 | Hartley RW. |
タイトル | Barnase and barstar. Expression of its cloned inhibitor permits expression of a cloned ribonuclease. |
ジャーナル | J Mol Biol |
Abstract |
Barnase is the extracellular ribonuclease of Bacillus amyloliquefaciens and barstar its specific intracellular inhibitor. The gene for barstar has now been cloned and sequenced. When the wild-type gene for barnase is reconstructed from its previously cloned parts on the same plasmid as the barstar gene, the lethal effect of its expression is suppressed. A plasmid has been devised which directs the secretion of 100 mg per active barnase liter by Escherichia coli and another which provides large (500 to 1000 mg/l) yields of barstar. The structure of these plasmids and the derived 89 amino acid sequence of barstar are reported. |
巻・号 | 202(4) |
ページ | 913-5 |
公開日 | 1988-8-20 |
DOI | 10.1016/0022-2836(88)90568-2 |
PII | 0022-2836(88)90568-2 |
PMID | 3050134 |
MeSH | Amino Acid Sequence Bacillus / genetics Bacterial Proteins / genetics* Base Sequence Cloning, Molecular* DNA, Bacterial Escherichia coli / genetics Genes, Bacterial Molecular Sequence Data Plasmids Ribonucleases / antagonists & inhibitors Ribonucleases / genetics* |
IF | 4.76 |
引用数 | 224 |
WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
リソース情報 | |
原核生物(大腸菌) | pMT416 |