Reference - Detail
| RRC ID | 33483 |
|---|---|
| Author | Hartley RW. |
| Title | Barnase and barstar. Expression of its cloned inhibitor permits expression of a cloned ribonuclease. |
| Journal | J Mol Biol |
| Abstract |
Barnase is the extracellular ribonuclease of Bacillus amyloliquefaciens and barstar its specific intracellular inhibitor. The gene for barstar has now been cloned and sequenced. When the wild-type gene for barnase is reconstructed from its previously cloned parts on the same plasmid as the barstar gene, the lethal effect of its expression is suppressed. A plasmid has been devised which directs the secretion of 100 mg per active barnase liter by Escherichia coli and another which provides large (500 to 1000 mg/l) yields of barstar. The structure of these plasmids and the derived 89 amino acid sequence of barstar are reported. |
| Volume | 202(4) |
| Pages | 913-5 |
| Published | 1988-8-20 |
| DOI | 10.1016/0022-2836(88)90568-2 |
| PII | 0022-2836(88)90568-2 |
| PMID | 3050134 |
| MeSH | Amino Acid Sequence Bacillus / genetics Bacterial Proteins / genetics* Base Sequence Cloning, Molecular* DNA, Bacterial Escherichia coli / genetics Genes, Bacterial Molecular Sequence Data Plasmids Ribonucleases / antagonists & inhibitors Ribonucleases / genetics* |
| IF | 4.76 |
| Times Cited | 224 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Prokaryotes E. coli | pMT416 |