論文 - 詳細
| RRC ID | 33492 |
|---|---|
| 著者 | Twigg AJ, Sherratt D. |
| タイトル | Trans-complementable copy-number mutants of plasmid ColE1. |
| ジャーナル | Nature |
| Abstract |
Plasmid ColE1, like many other small non-conjugative plasmids, is present in multiple copies (about 15 per chromsome equivalent) in Escherichia coli cells. Because of their high copy number, the replication of such plasmids has been described as 'relaxed', even though there is good evidence that it is strictly controlled: ColE1 derivatives have characteristic but different copy numbers and ColE1 copy-number mutants have been characterised. No plasmid-specified protein is essential for the replication of ColE1 and related plasmids, as extensive replication can occur in chloramphenicol-treated cells, in plasmid-free chloramphenicol-treated cells transfected with a hybrid ColE1/phage replicon and in vitro in extracts derived from plasmid-free cells. Nevertheless, it is possible that a plasmid-specified protein is involved in ColE1 replication control in viable cells. Here we show that deletion of a given non-essential region from ColE1-like plasmids results in a raised copy number. Such plasmids are stably maintained and have their copy number returned to normal when a complementing plasmid is present in the same cell, indicating that a plasmid-specified diffusible gene product regulates the plasmid content of ColE1-containing cells. Deletion of the equivalent region from the cloning vector pBR322 gives a derivative which has a raised copy number and which has also lost its origin for conjugal transfer; unlike pBR322, it cannot be mobilised. |
| 巻・号 | 283(5743) |
| ページ | 216-8 |
| 公開日 | 1980-1-10 |
| DOI | 10.1038/283216a0 |
| PMID | 7350544 |
| MeSH | Bacterial Proteins / genetics Bacteriocin Plasmids* Chloramphenicol / pharmacology Conjugation, Genetic DNA Replication* Drug Resistance, Microbial Gene Amplification Genetic Complementation Test Mutation Plasmids* Repressor Proteins / genetics* Transcription Factors / genetics* |
| IF | 42.779 |
| 引用数 | 957 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 29 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | pAT153 |