論文 - 詳細
| RRC ID | 33494 |
|---|---|
| 著者 | Nilsson B, Uhlén M, Josephson S, Gatenbeck S, Philipson L. |
| タイトル | An improved positive selection plasmid vector constructed by oligonucleotide mediated mutagenesis. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
An Escherichia coli plasmid vector, pUN121, has been constructed which allows for positive selection of transformants harboring DNA inserts. The positive selection of transformants harboring DNA inserts. The vector is based on plasmid pTR262 (Roberts et al. Gene, 12, (1980), 123-127) in which the tetracycline resistance gene is under transcriptional control of the repressor protein coded by the phage lambda cI gene. This plasmid has been rearranged, using in vitro recombinant techniques including oligonucleotide mediated mutagenesis to yield a smaller plasmid (4.4 kb) with unique cloning sites for EcoRI, XmaI and SmaI in addition to the unique HindIII and BclI sites. The plasmid has a functional ampicillin resistance gene and the new restriction sites (EcoRI, XmaI and SmaI) when used for cloning, give rise to tetracycline resistant transformants. |
| 巻・号 | 11(22) |
| ページ | 8019-30 |
| 公開日 | 1983-11-25 |
| DOI | 10.1093/nar/11.22.8019 |
| PMID | 6316281 |
| PMC | PMC326556 |
| MeSH | Ampicillin / toxicity Bacteriophage lambda / genetics Base Sequence DNA Transposable Elements Escherichia coli / genetics* Genes, Bacterial Genes, Viral Genetic Vectors* Mutation* Oligodeoxyribonucleotides / chemical synthesis Oligodeoxyribonucleotides / genetics* Oligonucleotides / genetics* Penicillin Resistance Plasmids* Transcription, Genetic |
| IF | 11.502 |
| 引用数 | 147 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 30 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | pUN122 |