Reference - Detail
| RRC ID | 33501 |
|---|---|
| Author | Büssow K, Cahill D, Nietfeld W, Bancroft D, Scherzinger E, Lehrach H, Walter G. |
| Title | A method for global protein expression and antibody screening on high-density filters of an arrayed cDNA library. |
| Journal | Nucleic Acids Res |
| Abstract |
We have developed a technique to establish catalogues of protein products of arrayed cDNA clones identified by DNA hybridisation or sequencing. A human fetal brain cDNA library was directionally cloned in a bacterial vector that allows IPTG-inducible expression of His6-tagged fusion proteins. Using robot technology, the library was arrayed in microtitre plates and gridded onto high-density in situ filters. A monoclonal antibody recognising the N-terminal RGSH6sequence of expressed proteins (RGS.His antibody, Qiagen) detected 20% of the library as putative expression clones. Two example genes, GAPDH and HSP90alpha, were identified on high-density filters using DNA probes and antibodies against their proteins. |
| Volume | 26(21) |
| Pages | 5007-8 |
| Published | 1998-11-1 |
| DOI | 10.1093/nar/26.21.5007 |
| PII | gkb763 |
| PMID | 9776767 |
| PMC | PMC147919 |
| MeSH | Antibodies, Monoclonal* Brain Chemistry Cloning, Molecular DNA, Complementary / genetics* Fetus Gene Expression Gene Library* Humans Molecular Sequence Data Nerve Tissue Proteins / genetics Nerve Tissue Proteins / immunology Proteins / genetics* Proteins / immunology* Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / immunology |
| IF | 11.502 |
| Times Cited | 207 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 79 |
| Altmetric score changes over past 6months | 3.0 |
| Resource | |
| Prokaryotes E. coli | pQE30NST pQStrep2 pQStrep4 pQTEV pSE111 |