論文 - 詳細
| RRC ID | 33511 |
|---|---|
| 著者 | Phillips GJ. |
| タイトル | New cloning vectors with temperature-sensitive replication. |
| ジャーナル | Plasmid |
| Abstract |
A series of cloning vectors with conditional, temperature-sensitive replication that are selectable with ampicillin, chloramphenicol, and kanamycin has been constructed. These vectors are derivatives of a pSC101 mutant that can replicate only at low temperatures. The cloning vectors carry a number of unique restriction sites and provide for screening of recombinant plasmids by alpha complementation. These vectors have proven useful for a variety of applications where conditional replication of a recombinant plasmid is desired. |
| 巻・号 | 41(1) |
| ページ | 78-81 |
| 公開日 | 1999-1-1 |
| DOI | 10.1006/plas.1998.1380 |
| PII | S0147-619X(98)91380-4 |
| PMID | 9887310 |
| MeSH | Cloning, Molecular / methods* DNA Replication* Escherichia coli / genetics Genetic Complementation Test Genetic Vectors / genetics* Genetic Vectors / metabolism Plasmids / genetics* Plasmids / metabolism Restriction Mapping Temperature |
| IF | 2.805 |
| 引用数 | 33 |
| WOS 分野 | GENETICS & HEREDITY MICROBIOLOGY |
| リソース情報 | |
| 原核生物(大腸菌) | pTSA30 pTSC30 pTSK30 |