論文 - 詳細
| RRC ID | 33538 |
|---|---|
| 著者 | Yoshida K, Yamamoto Y, Omae K, Yamamoto M, Fujita Y. |
| タイトル | Identification of two myo-inositol transporter genes of Bacillus subtilis. |
| ジャーナル | J Bacteriol |
| Abstract |
Among hundreds of mutants constructed systematically by the Japanese groups participating in the functional analysis of the Bacillus subtilis genome project, we found that a mutant with inactivation of iolT (ydjK) exhibited a growth defect on myo-inositol as the sole carbon source. The putative product of iolT exhibits significant similarity with many bacterial sugar transporters in the databases. In B. subtilis, the iolABCDEFGHIJ and iolRS operons are known to be involved in inositol utilization, and its transcription is regulated by the IolR repressor and induced by inositol. Among the iol genes, iolF was predicted to encode an inositol transporter. Inactivation of iolF alone did not cause such an obvious growth defect on inositol as the iolT inactivation, while simultaneous inactivation of the two genes led to a more severe defect than the single iolT inactivation. Determination of inositol uptake by the mutants revealed that iolT inactivation almost completely abolished uptake, but uptake by IolF itself was slightly detectable. These results, as well as the K(m) and V(max) values for the IolT and IolF inositol transporters, indicated that iolT and iolF encode major and minor inositol transporters, respectively. Northern and primer extension analyses of iolT transcription revealed that the gene is monocistronically transcribed from a promoter likely recognized by final sigma(A) RNA polymerase and negatively regulated by IolR as well. The interaction between IolR and the iolT promoter region was analyzed by means of gel retardation and DNase I footprinting experiments, it being suggested that the mode of interaction is quite similar to that found for the promoter regions of the iol divergon. |
| 巻・号 | 184(4) |
| ページ | 983-91 |
| 公開日 | 2002-2-1 |
| DOI | 10.1128/jb.184.4.983-991.2002 |
| PMID | 11807058 |
| PMC | PMC134797 |
| MeSH | Bacillus subtilis / genetics* Bacillus subtilis / growth & development Bacillus subtilis / metabolism Bacterial Proteins / genetics* Bacterial Proteins / metabolism Base Sequence Binding Sites Carbon Culture Media DNA, Bacterial DNA-Binding Proteins* Gene Expression Regulation, Bacterial Genes, Bacterial* Inositol / metabolism* Molecular Sequence Data Monosaccharide Transport Proteins / genetics* Monosaccharide Transport Proteins / metabolism Promoter Regions, Genetic Repressor Proteins / metabolism Transcription, Genetic |
| IF | 3.006 |
| 引用数 | 41 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(枯草菌) | MBS213 MBS214 MBS215 MBS216 MBS217 |