RRC ID |
33558
|
著者 |
Nanamiya H, Kasai K, Nozawa A, Yun CS, Narisawa T, Murakami K, Natori Y, Kawamura F, Tozawa Y.
|
タイトル |
Identification and functional analysis of novel (p)ppGpp synthetase genes in Bacillus subtilis.
|
ジャーナル |
Mol Microbiol
|
Abstract |
Bacterial alarmone (p)ppGpp, is a global regulator responsible for the stringent control. Two homologous (p)ppGpp synthetases, RelA and SpoT, have been identified and characterized in Escherichia coli, whereas Gram-positive bacteria such as Bacillus subtilis have been thought to possess only a single RelA-SpoT enzyme. We have now identified two genes, yjbM and ywaC, in B. subtilis that encode a novel type of alarmone synthetase. The predicted products of these genes are relatively small proteins ( approximately 25 kDa) that correspond to the (p)ppGpp synthetase domain of RelA-SpoT family members. A database survey revealed that genes homologous to yjbM and ywaC are conserved in certain bacteria belonging to Firmicutes or Actinobacteria phyla but not in other phyla such as Proteobacteria. We designated the proteins as small alarmone synthetases (SASs) to distinguish them from RelA-SpoT proteins. The (p)ppGpp synthetase function of YjbM and YwaC was confirmed by genetic complementation analysis and by in vitro assay of enzyme activity. Molecular genetic analysis also revealed that ywaC is induced by alkaline shock, resulting in the transient accumulation of ppGpp. The SAS proteins thus likely function in the biosynthesis of alarmone with a mode of action distinct from that of RelA-SpoT homologues.
|
巻・号 |
67(2)
|
ページ |
291-304
|
公開日 |
2008-1-1
|
DOI |
10.1111/j.1365-2958.2007.06018.x
|
PII |
MMI6018
|
PMID |
18067544
|
MeSH |
Amino Acid Sequence
Arginine / analogs & derivatives
Arginine / metabolism
Bacillus subtilis / classification
Bacillus subtilis / enzymology
Bacillus subtilis / genetics*
Bacterial Proteins / chemistry
Bacterial Proteins / genetics*
Bacterial Proteins / metabolism*
Escherichia coli / genetics
Escherichia coli Proteins / chemistry
Escherichia coli Proteins / genetics
Escherichia coli Proteins / metabolism
Gene Expression Regulation, Bacterial
Genetic Complementation Test
Guanosine Tetraphosphate / biosynthesis
Ligases / chemistry
Ligases / genetics*
Ligases / metabolism*
Ligases / physiology
Molecular Sequence Data
Mutation
Phylogeny
Sequence Alignment
Transcription, Genetic
|
IF |
3.418
|
引用数 |
135
|
WOS 分野
|
MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
原核生物(枯草菌) |
MBS8
MBS9
MBS10
MBS11
MBS12
MBS13
MBS14 |