RRC ID 33561
Author Yamamoto H, Hashimoto M, Higashitsuji Y, Harada H, Hariyama N, Takahashi L, Iwashita T, Ooiwa S, Sekiguchi J.
Title Post-translational control of vegetative cell separation enzymes through a direct interaction with specific inhibitor IseA in Bacillus subtilis.
Journal Mol Microbiol
Abstract Three D,L-endopeptidases, LytE, LytF and CwlS, are involved in the vegetative cell separation in Bacillus subtilis. A novel cell surface protein, IseA, inhibits the cell wall lytic activities of these d,l-endopeptidases in vitro, and IseA negatively regulates the cell separation enzymes at the post-translational level. Immunofluorescence microscopy indicated that the IseA-3xFLAG fusion protein was specifically localized at cell separation sites and poles on the vegetative cell surface in a similar manner of the d,l-endopeptidases. Furthermore, pull-down assay showed that IseA binds to the catalytic domain of LytF, indicating that IseA is localized on the cell surface through the catalytic domain of LytF. Overexpression of IseA caused a long-chained cell morphology in the exponential growth phase, indicating that IseA inhibits the cell separation D,L-endopeptidases in vivo. Besides, overexpression of IseA in a cwlO disruptant affected cell growth, implying that IseA is also involved in the cell elongation event. However, although IseA inhibits the activities of LytE, LytF, CwlS and CwlO in vitro, it is unlikely to inhibit CwlS and CwlO in vivo. This is the first demonstration that the cell separation event is post-translationally controlled through a direct interaction between cell separation enzymes and a specific novel inhibitor in bacteria.
Volume 70(1)
Pages 168-82
Published 2008-10-1
DOI 10.1111/j.1365-2958.2008.06398.x
PII MMI6398
PMID 18761694
MeSH Bacillus subtilis / enzymology* Bacillus subtilis / genetics Bacillus subtilis / growth & development Bacterial Proteins / metabolism* Carrier Proteins / metabolism* Catalytic Domain Cell Wall / enzymology* DNA, Bacterial / genetics Endopeptidases / metabolism* Enzyme Inhibitors / metabolism Genes, Bacterial Membrane Proteins / metabolism Microscopy, Fluorescence Plasmids Protein Processing, Post-Translational*
IF 3.649
Times Cited 24
WOS Category MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Prokaryotes B. subtilis MBS39 MBS40 MBS41 MBS42 MBS43 MBS44 MBS45 MBS46 MBS47