| RRC ID |
33566
|
| 著者 |
Nanamiya H, Sato M, Masuda K, Sato M, Wada T, Suzuki S, Natori Y, Katano M, Akanuma G, Kawamura F.
|
| タイトル |
Bacillus subtilis mutants harbouring a single copy of the rRNA operon exhibit severe defects in growth and sporulation.
|
| ジャーナル |
Microbiology (Reading)
|
| Abstract |
The number of copies of rRNA genes in bacterial genomes differs greatly among bacterial species. It is difficult to determine the functional significance of the heterogeneity of each rRNA operon fully due to the existence of multiple rRNA operons and because the sequence heterogeneity among the rRNA genes is extremely low. To overcome this problem, we sequentially deleted the ten rrn operons of Bacillus subtilis and constructed seven mutant strains that each harboured a single rrn operon (either rrnA, B, D, E, I, J or O) in their genome. The growth rates and sporulation frequencies of these mutants were reduced drastically compared with those of the wild-type strain, and this was probably due to decreased levels of ribosomes in the mutants. Interestingly, the ability to sporulate varied significantly among the mutant strains. These mutants have proved to be invaluable in our initial attempts to reveal the functional significance of the heterogeneity of each rRNA operon.
|
| 巻・号 |
156(Pt 10)
|
| ページ |
2944-2952
|
| 公開日 |
2010-10-1
|
| DOI |
10.1099/mic.0.035295-0
|
| PMID |
20634236
|
| MeSH |
Bacillus subtilis / genetics*
Bacillus subtilis / growth & development*
Bacillus subtilis / physiology
Gene Deletion
Gene Dosage
Mutation
Ribosomes / genetics
Spores, Bacterial / growth & development
rRNA Operon*
|
| IF |
2.138
|
| 引用数 |
21
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 原核生物(枯草菌) |
MBS1
MBS2
MBS3
MBS4
MBS5
MBS6
MBS7 |
