RRC ID 33568
Author Miwa Y, Fujita Y.
Title Determination of the cis sequence involved in catabolite repression of the Bacillus subtilis gnt operon; implication of a consensus sequence in catabolite repression in the genus Bacillus.
Journal Nucleic Acids Res
Abstract The mechanism underlying catabolite repression in Bacillus species remains unsolved. The gluconate (gnt) operon of Bacillus subtilis is one of the catabolic operons which is under catabolite repression. To identify the cis sequence involved in catabolite repression of the gnt operon, we performed deletion analysis of a DNA fragment carrying the gnt promoter and the gntR gene, which had been cloned into the promoter probe vector, pWP19. Deletion of the region upstream of the gnt promoter did not affect catabolite repression. Further deletion analysis of the gnt promoter and gntR coding region was carried out after restoration of promoter activity through the insertion of internal constitutive promoters of the gnt operon before the gntR gene (P2 and P3). These deletions revealed that the cis sequence involved in catabolite repression of the gnt operon is located between nucleotide positions +137 and +148. This DNA segment contains a sequence, ATTGAAAG, which may be implicated as a consensus sequence involved in catabolite repression in the genus Bacillus.
Volume 18(23)
Pages 7049-53
Published 1990-12-11
DOI 10.1093/nar/18.23.7049
PMID 2124676
PMC PMC332768
MeSH Bacillus subtilis / genetics* Bacillus subtilis / metabolism Base Sequence Chromosome Deletion Chromosomes, Bacterial Consensus Sequence* Gene Expression Regulation* Genes, Bacterial Gluconates / metabolism* Molecular Sequence Data Operon* Promoter Regions, Genetic Regulatory Sequences, Nucleic Acid*
IF 11.147
Times Cited 34
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Prokaryotes B. subtilis MBS135 MBS137