論文 - 詳細
| RRC ID | 33575 |
|---|---|
| 著者 | Akanuma G, Nanamiya H, Natori Y, Yano K, Suzuki S, Omata S, Ishizuka M, Sekine Y, Kawamura F. |
| タイトル | Inactivation of ribosomal protein genes in Bacillus subtilis reveals importance of each ribosomal protein for cell proliferation and cell differentiation. |
| ジャーナル | J Bacteriol |
| Abstract |
Among the 57 genes that encode ribosomal proteins in the genome of Bacillus subtilis, a Gram-positive bacterium, 50 genes were targeted by systematic inactivation. Individual deletion mutants of 16 ribosomal proteins (L1, L9, L15, L22, L23, L28, L29, L32, L33.1, L33.2, L34, L35, L36, S6, S20, and S21) were obtained successfully. In conjunction with previous reports, 22 ribosomal proteins have been shown to be nonessential in B. subtilis, at least for cell proliferation. Although several mutants that harbored a deletion of a ribosomal protein gene did not show any significant differences in any of the phenotypes that were tested, various mutants showed a reduced growth rate and reduced levels of 70S ribosomes compared with the wild type. In addition, severe defects in the sporulation frequency of the ΔrplA (L1) mutant and the motility of the ΔrpsU (S21) mutant were observed. These data provide the first evidence in B. subtilis that L1 and S21 are required for the progression of cellular differentiation. |
| 巻・号 | 194(22) |
| ページ | 6282-91 |
| 公開日 | 2012-11-1 |
| DOI | 10.1128/JB.01544-12 |
| PII | JB.01544-12 |
| PMID | 23002217 |
| PMC | PMC3486396 |
| MeSH | Bacillus subtilis / cytology Bacillus subtilis / genetics Bacillus subtilis / metabolism* Cell Proliferation* Gene Deletion* Gene Expression Regulation, Bacterial / physiology* Ribosomal Proteins / genetics Ribosomal Proteins / metabolism* Temperature Time Factors Transcriptome |
| IF | 3.006 |
| 引用数 | 55 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(枯草菌) | MBS15 MBS16 MBS17 MBS18 MBS19 MBS20 MBS21 MBS22 MBS23 MBS24 MBS25 MBS26 MBS27 MBS28 MBS29 MBS30 |