論文 - 詳細
| RRC ID | 33581 |
|---|---|
| 著者 | Fujita Y, Nihashi J, Fujita T. |
| タイトル | The characterization and cloning of a gluconate (gnt) operon of Bacillus subtilis. |
| ジャーナル | J Gen Microbiol |
| Abstract |
The enzymes involved in gluconate utilization in Bacillus subtilis seemed to be gluconate permease and gluconate kinase. Several mutants unable to grow on gluconate were isolated. The mutations they harboured (gnt) were clustered between iol-6 and fdp-74 on the B. subtilis chromosome (a tentative map order of gnt-10, gnt-4, gnt-26, gnt-23 and gnt-9 was obtained). The gnt-10 mutation seemed to be located within the structural gene of the kinase, and the gnt-23 and gnt-26 mutations seemed to be within that of the permease. An EcoRI fragment (4.5 MDal) containing an intact gluconate (gnt) operon consisting of these two structural genes was cloned in phage phi 105 by prophage transformation and was mapped physically. The physical location of the mutations coincided with their order on the genetic map. The HindIII-A fragment (2.4 MDal), which corrects all the gnt mutations, was subcloned in plasmid pC194. The fragment contained the structural genes for the gluconate permease and kinase, but not the regulatory region of the gluconate operon. |
| 巻・号 | 132(1) |
| ページ | 161-9 |
| 公開日 | 1986-1-1 |
| DOI | 10.1099/00221287-132-1-161 |
| PMID | 3011959 |
| MeSH | Bacillus subtilis / enzymology Bacillus subtilis / genetics* Bacteriophages / genetics Chromosome Mapping Cloning, Molecular* DNA Restriction Enzymes Electrophoresis, Agar Gel Enzyme Induction Gluconates / genetics* Membrane Transport Proteins / biosynthesis Mutation Operon* Phosphotransferases / biosynthesis Phosphotransferases (Alcohol Group Acceptor)* Transformation, Bacterial |
| 引用数 | 16 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 原核生物(枯草菌) | MBS83 MBS100 MBS101 MBS102 MBS103 MBS119 MBS120 MBS122 MBS123 MBS112 MBS113 MBS114 |