RRC ID 33582
Author Fujita Y, Fujita T, Miwa Y, Nihashi J, Aratani Y.
Title Organization and transcription of the gluconate operon, gnt, of Bacillus subtilis.
Journal J. Biol. Chem.
Abstract The gluconate (gnt) operon of Bacillus subtilis has been cloned and sequenced. Analysis of the sequence (5482 base pairs) revealed four open reading frames, each of which was preceded by a Shine-Dalgarno sequence. These four frames were designated from the 5'-end as gntR, gntK, gntP, and gntZ. The gntR and gntK genes overlapped by 5 bases. The gntK and gntP gene products (consisting of 513 and 448 amino acids) were identified as gluconate kinase and permease, respectively, by means of insertional inactivation and deletion analysis of these genes subcloned in plasmid pC194. The functions of the gntR and gntZ gene products (243 and 468 amino acids) are presently unknown. S1 nuclease mapping and subcloning in a promoter probe vector (pPL603B) provided evidence that the gnt operon was transcribed as a polycistronic mRNA. Besides the gnt promoter about 40 base pairs upstream of the gntR gene, we detected two overlapping internal promoters between the gntP and gntZ genes. The gnt transcripts terminate about 45 base pairs downstream of the gntZ gene.
Volume 261(29)
Pages 13744-53
Published 1986-10-15
PMID 3020045
MeSH Amino Acid Sequence Bacillus subtilis / genetics* Base Sequence Cloning, Molecular DNA Restriction Enzymes Escherichia coli Proteins Genes* Genes, Bacterial* Gluconates / metabolism* Membrane Transport Proteins / genetics Nucleic Acid Conformation Operon* Phosphotransferases / genetics Phosphotransferases (Alcohol Group Acceptor)* Plasmids Transcription, Genetic*
IF 4.106
Times Cited 100
Prokaryotes B. subtilis MBS105 MBS126 MBS127