RRC ID 33586
Author Fujita Y, Fujita T.
Title Genetic analysis of a pleiotropic deletion mutation (delta igf) in Bacillus subtilis.
Journal J. Bacteriol.
Abstract A delta igf mutation of Bacillus subtilis (formerly called fdpAl) is a large deletion causing pleiotropic defects. The mapping of the delta igf deletion by phage PBS1 transduction revealed the following map order: sacA, thiC, hsrE, delta igf, ts199, purA. To analyze the pleiotropic nature of the delta igf mutation, mutants affected in each property of the pleiotropic mutation were isolated, and the mutations were mapped. iol and gnt mutants could not grow on inositol and gluconate, respectively, and fdp mutants were affected only in fructose-bisphosphatase. The map order from sacA to purA was as follows: sacA, thiC, hsrE, iol-6, gnt-4, fdp-74, hsrB, ts199, purA. The delta igf deletion covered loci from iol-6 to hsrB.
Volume 154(2)
Pages 864-9
Published 1983-5
PMID 6302085
PMC PMC217540
MeSH Bacillus subtilis / genetics* Bacillus subtilis / metabolism Chromosome Mapping Chromosomes, Bacterial Fructose-Bisphosphatase / genetics* Fructose-Bisphosphatase / metabolism Genes, Bacterial* Gluconates / metabolism* Inositol / metabolism* Mutation
IF 3.234
Times Cited 34
Prokaryotes B. subtilis MBS76 MBS77 MBS78 MBS79 MBS80 MBS85 MBS86 MBS87 MBS88 MBS90 MBS91 MBS93 MBS95 MBS96 MBS98 MBS94 MBS89