RRC ID 33594
Author Fujita Y, Miwa Y, Galinier A, Deutscher J.
Title Specific recognition of the Bacillus subtilis gnt cis-acting catabolite-responsive element by a protein complex formed between CcpA and seryl-phosphorylated HPr.
Journal Mol. Microbiol.
Abstract Catabolite repression of various Bacillus subtilis catabolic operons which carry a cis-acting catabolite-responsive element (CRE), such as the gnt operon, is mediated by CcpA, a protein belonging to the GalR-Lacl family of bacterial transcriptional repressors/activators, and the seryl-phosphorylated form of HPr, a phosphocarrier protein of the phosphoenolpyruvate:sugar phosphotransferase system. Footprinting experiments revealed that the purified CcpA protein interacted with P-ser-HPr to cause specific protection of the gnt CRE against DNase I digestion. The specific recognition of the gnt CRE was confirmed by the results of footprinting experiments using mutant gnt CREs carrying one of the following base substitutions within the CRE consensus sequence: G to T at position +149 or C to T at position +154 (+1 is the gnt transcription initiation nucleotide). The two mutant CREs causing a partial relief from catabolite repression were not protected by the CcpA/P-ser-HPr complex in footprinting experiments. Based on these and previous findings, we propose a molecular mechanism underlying catabolite repression in B. subtilis mediated by CcpA and P-ser-HPr.
Volume 17(5)
Pages 953-60
Published 1995-9
PMID 8596444
MeSH Amino Acid Sequence Bacillus subtilis / genetics* Bacillus subtilis / metabolism* Bacterial Proteins / metabolism Base Sequence Binding Sites DNA Probes DNA, Bacterial / chemistry DNA, Bacterial / metabolism DNA-Binding Proteins / metabolism* Molecular Sequence Data Operon* Phosphoenolpyruvate Sugar Phosphotransferase System / metabolism* Phosphorylation Phosphoserine Promoter Regions, Genetic Recombinant Proteins / metabolism Repressor Proteins / metabolism* Restriction Mapping
IF 3.649
Times Cited 179
Prokaryotes B. subtilis MBS160 MBS161 MBS162