RRC ID 33601
Author Morimoto T, Ara K, Ozaki K, Ogasawara N.
Title A simple method for introducing marker-free deletions in the Bacillus subtilis genome.
Journal Methods Mol. Biol.
Abstract A genetic tool for introducing marker-free deletions is essential for multiple manipulations of genomes. We have developed a simple and efficient method for creating marker-free deletion mutants of Bacillus subtilis through transformation with recombinant PCR products, using the Escherichia coli mazF gene encoding an endoribonuclease that cleaves free mRNAs as a counterselection tool.
Volume 765
Pages 345-58
Published 2011
DOI 10.1007/978-1-61779-197-0_20
PMID 21815102
MeSH Bacillus subtilis / genetics* DNA, Recombinant / genetics* Endoribonucleases / genetics Endoribonucleases / metabolism Escherichia coli / enzymology Escherichia coli / genetics Gene Deletion* Genetic Markers / genetics Genome, Bacterial / genetics* Polymerase Chain Reaction Transformation, Bacterial / genetics*
Times Cited 3
WOS Category BIOCHEMICAL RESEARCH METHODS GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Prokaryotes B. subtilis TMO310 TMO311