Reference - Detail
RRC ID | 33601 |
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Author | Morimoto T, Ara K, Ozaki K, Ogasawara N. |
Title | A simple method for introducing marker-free deletions in the Bacillus subtilis genome. |
Journal | Methods Mol Biol |
Abstract |
A genetic tool for introducing marker-free deletions is essential for multiple manipulations of genomes. We have developed a simple and efficient method for creating marker-free deletion mutants of Bacillus subtilis through transformation with recombinant PCR products, using the Escherichia coli mazF gene encoding an endoribonuclease that cleaves free mRNAs as a counterselection tool. |
Volume | 765 |
Pages | 345-58 |
Published | 2011-1-1 |
DOI | 10.1007/978-1-61779-197-0_20 |
PMID | 21815102 |
MeSH | Bacillus subtilis / genetics* DNA, Recombinant / genetics* Endoribonucleases / genetics Endoribonucleases / metabolism Escherichia coli / enzymology Escherichia coli / genetics Gene Deletion* Genetic Markers / genetics Genome, Bacterial / genetics* Polymerase Chain Reaction Transformation, Bacterial / genetics* |
Times Cited | 6 |
WOS Category | BIOCHEMICAL RESEARCH METHODS GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY |
Resource | |
Prokaryotes B. subtilis | TMO310 TMO311 |