RRC ID 33602
Author Morimoto T, Ara K, Ozaki K, Ogasawara N.
Title A new simple method to introduce marker-free deletions in the Bacillus subtilis genome.
Journal Genes Genet Syst
Abstract A genetic tool to introduce marker-free deletions is essential for multiple manipulations of genomes. We report a simple and efficient method to create marker-free deletion mutants of Bacillus subtilis through transformation with recombinant PCR products, using the Escherichia coli mazF gene encoding an endoribonuclease that cleaves free mRNAs as a counter-selection tool. Our method will be applicable to any bacterium in which introduction of the mazF cassette into the genome by double crossover homologous recombination is possible.
Volume 84(4)
Pages 315-8
Published 2009-8-1
DOI 10.1266/ggs.84.315
PII JST.JSTAGE/ggs/84.315
PMID 20057169
MeSH Bacillus subtilis / genetics* DNA-Binding Proteins / genetics Endoribonucleases / genetics Escherichia coli / genetics Escherichia coli Proteins / genetics Gene Deletion* Genetic Markers / genetics Genome, Bacterial / genetics* Recombination, Genetic*
IF 0.917
Times Cited 29
Prokaryotes B. subtilis TMO310 TMO311