RRC ID 3363
Author Fujii T, Tanaka N, Yokoyama T, Ninaki O, Oshiki T, Ohnuma A, Tazima Y, Banno Y, Ajimura M, Mita K, Seki M, Ohbayashi F, Shimada T, Abe H.
Title The female-killing chromosome of the silkworm, Bombyx mori, was generated by translocation between the Z and W chromosomes.
Journal Genetica
Abstract Bombyx mori is a female-heterogametic organism (female, ZW; male, ZZ) that appears to have a putative feminizing gene (Fem) on the W chromosome. The paternally transmitted mutant W chromosome, Df(p ( Sa ) + ( p )W + ( od ))Fem, derived from the translocation-carrying W chromosome (p ( Sa ) + ( p )W + ( od )), is inert as femaleness determinant. Moreover, this Df(p ( Sa ) + ( p )W + ( od ))Fem chromosome has been thought to have a female-killing factor because no female larvae having the Df(p ( Sa ) + ( p )W + ( od ))Fem chromosome are produced. Initially, to investigate whether the Df(p ( Sa ) + ( p )W + ( od ))Fem chromosome contains any region of the W chromosome or not, we analyzed the presence or absence of 12 W-specific RAPD markers. The Df(p ( Sa ) + ( p )W + ( od ))Fem chromosome contained 3 of 12 W-specific RAPD markers. These results strongly indicate that the Df(p ( Sa ) + ( p )W + ( od ))Fem chromosome contains the region of the W chromosome. Moreover, by using phenotypic and molecular markers, we confirmed that the Df(p ( Sa ) + ( p )W + ( od ))Fem chromosome is connected with a partially deleted Z chromosome and that this fused chromosome behaves as a Z chromosome during male meiosis. Furthermore, we demonstrated that the ZZW-type triploid female having the Df(p ( Sa ) + ( p )W + ( od ))Fem chromosome is viable. Therefore, we concluded that the Df(p ( Sa ) + ( p )W + ( od ))Fem chromosome does not have a female-killing factor but that partial deletion of the Z chromosome causes the death of the ZW-type diploid female having the Df(p ( Sa ) + ( p )W + ( od ))Fem chromosome. Additionally, our results of detailed genetic analyses strongly indicate that the female-killing chromosome composed of the Df(p ( Sa ) + ( p )W + ( od ))Fem chromosome and deleted Z chromosome was generated by translocation between the Z chromosome and the translocation-carrying W chromosome, p ( Sa ) + ( p )W + ( od ).
Volume 127(1-3)
Pages 253-65
Published 2006-5
DOI 10.1007/s10709-005-4147-8
PMID 16850229
MeSH Animals Animals, Inbred Strains Bombyx / embryology Bombyx / genetics* Chromosome Breakage Chromosome Deletion Eggs Female Feminization / genetics Genes, Lethal* Genetic Markers Genotype Male Polymorphism, Restriction Fragment Length Polyploidy Sex Characteristics Sex Chromosome Aberrations* Sex Determination Analysis Survival Analysis Translocation, Genetic / physiology*
IF 1.366
Times Cited 10
WOS Category GENETICS & HEREDITY
Resource
Silkworms p50