論文 - 詳細
| RRC ID | 33648 |
|---|---|
| 著者 | Fitzpatrick P, Shattil SJ, Ablooglu AJ. |
| タイトル | C-terminal COOH of integrin β1 is necessary for β1 association with the kindlin-2 adapter protein. |
| ジャーナル | J Biol Chem |
| Abstract |
Protein-protein interactions are driving forces in cellular processes. As a prime example, transmembrane integrins link extracellular matrix and intracellular proteins, resulting in bidirectional signaling that regulates cell migration, proliferation, differentiation, and survival. Here we provide the first evidence that interaction between the integrin β1 cytoplasmic tail and kindlin-2, a member of a family of adapters implicated in human disease pathogenesis, is mainly governed by the β1 C-terminal carboxylate moiety and is required for laterality organ development in zebrafish. Affinity measurements indicate that this unusual protein-protein interaction mode is coordinated by a putative carboxylate-binding motif in the kindlin-2 FERM subdomain F3. Contrary to the C terminus of proteins that engage PDZ domains, the C-terminal three residues of β1, per se, do not contribute to kindlin-2 binding or to laterality organ development. Thus, by employing zebrafish as an in situ physiological tool to correlate protein structure and function, we have discovered an unexpected association chemistry between an integrin and a key adapter involved in integrin signaling. |
| 巻・号 | 289(16) |
| ページ | 11183-11193 |
| 公開日 | 2014-4-18 |
| DOI | 10.1074/jbc.M113.535369 |
| PII | S0021-9258(20)48336-X |
| PMID | 24599960 |
| PMC | PMC4036257 |
| MeSH | Animals Human Umbilical Vein Endothelial Cells Humans Integrin beta1 / genetics Integrin beta1 / metabolism* Membrane Proteins / genetics Membrane Proteins / metabolism* Neoplasm Proteins / genetics Neoplasm Proteins / metabolism* Protein Structure, Tertiary Signal Transduction / physiology* Zebrafish / embryology Zebrafish / genetics Zebrafish / metabolism* Zebrafish Proteins / genetics Zebrafish Proteins / metabolism* |
| IF | 4.238 |
| 引用数 | 7 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | Tg(sox17:EGFP)/ha01 |