RRC ID 33658
著者 Cheng YC, Scotting PJ, Hsu LS, Lin SJ, Shih HY, Hsieh FY, Wu HL, Tsao CL, Shen CJ.
タイトル Zebrafish rgs4 is essential for motility and axonogenesis mediated by Akt signaling.
ジャーナル Cell Mol Life Sci
Abstract The schizophrenia susceptibility gene, Rgs4, is one of the most intensively studied regulators of G-protein signaling members, well known to be fundamental in regulating neurotransmission. However, little is known about its role in the developing nervous system. We have isolated zebrafish rgs4 and shown that it is transcribed in the developing nervous system. Rgs4 knockdown did not affect neuron number and patterning but resulted in locomotion defects and aberrant development of axons. This was confirmed using a selective Rgs4 inhibitor, CCG-4986. Rgs4 knockdown also attenuated the level of phosphorylated-Akt1, and injection of constitutively-activated AKT1 rescued the motility defects and axonal phenotypes in the spinal cord but not in the hindbrain and trigeminal neurons. Our in vivo analysis reveals a novel role for Rgs4 in regulating axonogenesis during embryogenesis, which is mediated by another schizophrenia-associated gene, Akt1, in a region-specific manner.
巻・号 70(5)
ページ 935-50
公開日 2013-3-1
DOI 10.1007/s00018-012-1178-z
PMID 23052218
MeSH Amino Acid Sequence Animals Axons / metabolism* Axons / pathology* Gene Expression Regulation, Developmental Gene Knockdown Techniques Humans Molecular Sequence Data Nervous System / embryology Nervous System / metabolism Nervous System / pathology Neurogenesis Neurons / cytology* Neurons / metabolism Neurons / pathology Phylogeny Proto-Oncogene Proteins c-akt / metabolism* RGS Proteins / chemistry RGS Proteins / genetics RGS Proteins / metabolism* Sequence Alignment Signal Transduction Zebrafish / embryology Zebrafish / genetics Zebrafish / metabolism*
IF 6.496
引用数 22
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
ゼブラフィッシュ Tg(isl1:GFP)/rw0