論文 - 詳細
| RRC ID | 33670 |
|---|---|
| 著者 | Ye D, Lin F. |
| タイトル | S1pr2/Gα13 signaling controls myocardial migration by regulating endoderm convergence. |
| ジャーナル | Development |
| Abstract |
A key process during vertebrate heart development is the migration of bilateral populations of myocardial precursors towards the midline to form the primitive heart tube. In zebrafish, signaling mediated by sphingosine-1-phosphate (S1P) and its cognate G protein-coupled receptor (S1pr2/Mil) is essential for myocardial migration, but the underlying mechanisms remain undefined. Here, we show that suppression of Gα(13) signaling disrupts myocardial migration, leading to the formation of two bilaterally located hearts (cardia bifida). Genetic studies indicate that Gα(13) acts downstream of S1pr2 to regulate myocardial migration through a RhoGEF-dependent pathway. Furthermore, disrupting any component of the S1pr2/Gα(13)/RhoGEF pathway impairs endoderm convergence during segmentation, and the endodermal defects correlate with the extent of cardia bifida. Moreover, endoderm transplantation reveals that the presence of wild-type anterior endodermal cells in Gα(13)-deficient embryos is sufficient to rescue the endoderm convergence defect and cardia bifida, and, conversely, that the presence of anterior endodermal cells defective for S1pr2 or Gα(13) in wild-type embryos causes such defects. Thus, S1pr2/Gα(13) signaling probably acts in the endoderm to regulate myocardial migration. In support of this notion, cardiac-specific expression of Gα(13) fails to rescue cardia bifida in the context of global Gα(13) inhibition. Our data demonstrate for the first time that the Gα(13)/RhoGEF-dependent pathway functions downstream of S1pr2 to regulate convergent movement of the endoderm, an event that is crucial for coordinating myocardial migration. |
| 巻・号 | 140(4) |
| ページ | 789-99 |
| 公開日 | 2013-2-1 |
| DOI | 10.1242/dev.085340 |
| PII | dev.085340 |
| PMID | 23318642 |
| PMC | PMC3557776 |
| MeSH | Animals Cell Movement / physiology* Endoderm / embryology* Fluorescent Antibody Technique GTP-Binding Protein alpha Subunits, G12-G13 / metabolism* Heart / embryology* In Situ Hybridization Morpholinos / genetics Myocardium / cytology* Receptors, Lysosphingolipid / metabolism* Signal Transduction / physiology* Time-Lapse Imaging Zebrafish / embryology* |
| IF | 5.611 |
| 引用数 | 27 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | Tg(sox17:EGFP)/ha01 |