RRC ID |
3369
|
著者 |
Niimi T, Sahara K, Oshima H, Yasukochi Y, Ikeo K, Traut W.
|
タイトル |
Molecular cloning and chromosomal localization of the Bombyx Sex-lethal gene.
|
ジャーナル |
Genome
|
Abstract |
We cloned Bm-Sxl, an orthologue of the Drosophila melanogaster Sex-lethal (Sxl) gene from embryos of Bombyx mori. The full-length cDNAs were of 2 sizes, 1528 and 1339 bp, and were named Bm-Sxl-L and Bm-Sxl-S, respectively. Bm-Sxl-L consists of 8 exons and spans more than 20 kb of genomic DNA. The open reading frame (ORF) codes for a protein 336 amino acids in length. Bm-Sxl-S is a splice variant that lacks the second exon. This creates a new translation start 138 nucleotides downstream and an ORF that codes for 46 amino acids fewer at the N-terminus. Linkage analysis using an F2 panel mapped Bm-Sxl to linkage group 16 at 69.8 cM. We isolated 2 BACs that include the Bm-Sxl gene. With BAC-FISH we located Bm-Sxl cytogenetically on the chromosome corresponding to linkage group 16 (LG16) at position >68.8 cM.
|
巻・号 |
49(3)
|
ページ |
263-8
|
公開日 |
2006-3-1
|
DOI |
10.1139/g05-108
|
PII |
g05-108
|
PMID |
16604109
|
MeSH |
Alternative Splicing
Amino Acid Sequence
Animals
Base Sequence
Bombyx / embryology
Bombyx / genetics*
Chromosome Mapping
Chromosomes*
Cloning, Molecular*
DNA / genetics
DNA, Complementary / genetics
Embryo, Nonmammalian
Exons
Genes, Insect*
Genetic Linkage
Genome
In Situ Hybridization, Fluorescence
Insect Proteins / chemistry*
Molecular Sequence Data
Open Reading Frames
Protein Isoforms
|
IF |
2.037
|
引用数 |
32
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
GENETICS & HEREDITY
|
リソース情報 |
カイコ |
p50
No.459 ? |