論文 - 詳細
| RRC ID | 3369 |
|---|---|
| 著者 | Niimi T, Sahara K, Oshima H, Yasukochi Y, Ikeo K, Traut W. |
| タイトル | Molecular cloning and chromosomal localization of the Bombyx Sex-lethal gene. |
| ジャーナル | Genome |
| Abstract |
We cloned Bm-Sxl, an orthologue of the Drosophila melanogaster Sex-lethal (Sxl) gene from embryos of Bombyx mori. The full-length cDNAs were of 2 sizes, 1528 and 1339 bp, and were named Bm-Sxl-L and Bm-Sxl-S, respectively. Bm-Sxl-L consists of 8 exons and spans more than 20 kb of genomic DNA. The open reading frame (ORF) codes for a protein 336 amino acids in length. Bm-Sxl-S is a splice variant that lacks the second exon. This creates a new translation start 138 nucleotides downstream and an ORF that codes for 46 amino acids fewer at the N-terminus. Linkage analysis using an F2 panel mapped Bm-Sxl to linkage group 16 at 69.8 cM. We isolated 2 BACs that include the Bm-Sxl gene. With BAC-FISH we located Bm-Sxl cytogenetically on the chromosome corresponding to linkage group 16 (LG16) at position >68.8 cM. |
| 巻・号 | 49(3) |
| ページ | 263-8 |
| 公開日 | 2006-3-1 |
| DOI | 10.1139/g05-108 |
| PII | g05-108 |
| PMID | 16604109 |
| MeSH | Alternative Splicing Amino Acid Sequence Animals Base Sequence Bombyx / embryology Bombyx / genetics* Chromosome Mapping Chromosomes* Cloning, Molecular* DNA / genetics DNA, Complementary / genetics Embryo, Nonmammalian Exons Genes, Insect* Genetic Linkage Genome In Situ Hybridization, Fluorescence Insect Proteins / chemistry* Molecular Sequence Data Open Reading Frames Protein Isoforms |
| IF | 2.037 |
| 引用数 | 32 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| カイコ | p50 No.459 ? |