RRC ID 3369
Author Niimi T, Sahara K, Oshima H, Yasukochi Y, Ikeo K, Traut W.
Title Molecular cloning and chromosomal localization of the Bombyx Sex-lethal gene.
Journal Genome
Abstract We cloned Bm-Sxl, an orthologue of the Drosophila melanogaster Sex-lethal (Sxl) gene from embryos of Bombyx mori. The full-length cDNAs were of 2 sizes, 1528 and 1339 bp, and were named Bm-Sxl-L and Bm-Sxl-S, respectively. Bm-Sxl-L consists of 8 exons and spans more than 20 kb of genomic DNA. The open reading frame (ORF) codes for a protein 336 amino acids in length. Bm-Sxl-S is a splice variant that lacks the second exon. This creates a new translation start 138 nucleotides downstream and an ORF that codes for 46 amino acids fewer at the N-terminus. Linkage analysis using an F2 panel mapped Bm-Sxl to linkage group 16 at 69.8 cM. We isolated 2 BACs that include the Bm-Sxl gene. With BAC-FISH we located Bm-Sxl cytogenetically on the chromosome corresponding to linkage group 16 (LG16) at position >68.8 cM.
Volume 49(3)
Pages 263-8
Published 2006-3
DOI 10.1139/g05-108
PII g05-108
PMID 16604109
MeSH Alternative Splicing Amino Acid Sequence Animals Base Sequence Bombyx / embryology Bombyx / genetics* Chromosome Mapping Chromosomes* Cloning, Molecular* DNA / genetics DNA, Complementary / genetics Embryo, Nonmammalian Exons Genes, Insect* Genetic Linkage Genome In Situ Hybridization, Fluorescence Insect Proteins / chemistry* Molecular Sequence Data Open Reading Frames Protein Isoforms
IF 2.152
Times Cited 27
WOS Category BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY
Resource
Silkworms p50 No.459 ?