RRC ID 33707
Author Katagiri N, Kuroda T, Kishimoto H, Hayashi Y, Kumazawa T, Kimura K.
Title The nucleolar protein nucleophosmin is essential for autophagy induced by inhibiting Pol I transcription.
Journal Sci Rep
Abstract Various cellular stresses activate autophagy, which is involved in lysosomal degradation of cytoplasmic materials for maintaining nutrient homeostasis and eliminating harmful components. Here, we show that RNA polymerase I (Pol I) transcription inhibition induces nucleolar disruption and autophagy. Treatment with autophagy inhibitors or siRNA specific for autophagy-related (ATG) proteins inhibited autophagy but not nucleolar disruption induced by Pol I transcription inhibition, which suggested that nucleolar disruption was upstream of autophagy. Furthermore, treatment with siRNA specific for nucleolar protein nucleophosmin (NPM) inhibited this type of autophagy. This showed that NPM was involved in autophagy when the nucleolus was disrupted by Pol I inhibition. In contrast, NPM was not required for canonical autophagy induced by nutrient starvation, as it was not accompanied by nucleolar disruption. Thus, our results revealed that, in addition to canonical autophagy, there may be NPM-dependent autophagy associated with nucleolar disruption.
Volume 5
Pages 8903
Published 2015-3-10
DOI 10.1038/srep08903
PII srep08903
PMID 25754892
PMC PMC4354046
MeSH Autophagy / genetics* Cell Nucleolus / genetics Humans MCF-7 Cells Nuclear Proteins / antagonists & inhibitors Nuclear Proteins / genetics* Nucleolus Organizer Region / genetics Nucleophosmin RNA Polymerase I / antagonists & inhibitors RNA Polymerase I / genetics* RNA, Small Interfering Transcription, Genetic*
IF 3.998
Times Cited 13
DNA material pEGFP-C1-hLC3B (RDB13435)