RRC ID 33710
Author Morimoto T, Kadoya R, Endo K, Tohata M, Sawada K, Liu S, Ozawa T, Kodama T, Kakeshita H, Kageyama Y, Manabe K, Kanaya S, Ara K, Ozaki K, Ogasawara N.
Title Enhanced recombinant protein productivity by genome reduction in Bacillus subtilis.
Journal DNA Res.
Abstract The emerging field of synthetic genomics is expected to facilitate the generation of microorganisms with the potential to achieve a sustainable society. One approach towards this goal is the reduction of microbial genomes by rationally designed deletions to create simplified cells with predictable behavior that act as a platform to build in various genetic systems for specific purposes. We report a novel Bacillus subtilis strain, MBG874, depleted of 874 kb (20%) of the genomic sequence. When compared with wild-type cells, the regulatory network of gene expression of the mutant strain is reorganized after entry into the transition state due to the synergistic effect of multiple deletions, and productivity of extracellular cellulase and protease from transformed plasmids harboring the corresponding genes is remarkably enhanced. To our knowledge, this is the first report demonstrating that genome reduction actually contributes to the creation of bacterial cells with a practical application in industry. Further systematic analysis of changes in the transcriptional regulatory network of MGB874 cells in relation to protein productivity should facilitate the generation of improved B. subtilis cells as hosts of industrial protein production.
Volume 15(2)
Pages 73-81
Published 2008-4-30
DOI 10.1093/dnares/dsn002
PII dsn002
PMID 18334513
PMC PMC2650625
MeSH Bacillus subtilis / genetics* Bacillus subtilis / growth & development Bacillus subtilis / metabolism Bacterial Proteins / genetics Bacterial Proteins / metabolism Cellulases / genetics Cellulases / metabolism Endopeptidases / genetics Endopeptidases / metabolism Gene Deletion* Gene Expression Regulation, Bacterial* Genome, Bacterial* Genomics / methods Industrial Microbiology / methods* Recombinant Proteins / genetics Recombinant Proteins / metabolism* Spores, Bacterial
IF 5.415
Times Cited 77
WOS Category GENETICS & HEREDITY
Resource
Prokaryotes B. subtilis TMO310 TMO311 NED0100 NED0202 NED0802 NED0803 NED0804 NED0900 NED1400 NED1500 NED3402 NED0301 NED0302 NED0400 NED0501 NED0600 NED1002 NED1003 NED1100 NED1200 NED1602 NED1901 NED1902 NED2201 NED2202 NED2402 NED2602 NED2802 NED2900 NED4000 NED3000 NED3200 NED3301 NED3701 NED3800 NED1300 NED1802 NED2300 NED2701 NED3100 NED3303 NED4100 NED02021 NED02022 NED4001 NED4002 NED37022 NED0700 MGB469 MGB533 MGB559 MGB592 MGB604 MGB625 MGB723 MGB773 MGB822 MGB834 MGB860 MGB874