論文 - 詳細
| RRC ID | 3374 |
|---|---|
| 著者 | Audhya A, Hyndman F, McLeod IX, Maddox AS, Yates JR 3rd, Desai A, Oegema K. |
| タイトル | A complex containing the Sm protein CAR-1 and the RNA helicase CGH-1 is required for embryonic cytokinesis in Caenorhabditis elegans. |
| ジャーナル | J Cell Biol |
| Abstract |
Cytokinesis completes cell division and partitions the contents of one cell to the two daughter cells. Here we characterize CAR-1, a predicted RNA binding protein that is implicated in cytokinesis. CAR-1 localizes to germline-specific RNA-containing particles and copurifies with the essential RNA helicase, CGH-1, in an RNA-dependent fashion. The atypical Sm domain of CAR-1, which directly binds RNA, is dispensable for CAR-1 localization, but is critical for its function. Inhibition of CAR-1 by RNA-mediated depletion or mutation results in a specific defect in embryonic cytokinesis. This cytokinesis failure likely results from an anaphase spindle defect in which interzonal microtubule bundles that recruit Aurora B kinase and the kinesin, ZEN-4, fail to form between the separating chromosomes. Depletion of CGH-1 results in sterility, but partially depleted worms produce embryos that exhibit the CAR-1-depletion phenotype. Cumulatively, our results suggest that CAR-1 functions with CGH-1 to regulate a specific set of maternally loaded RNAs that is required for anaphase spindle structure and cytokinesis. |
| 巻・号 | 171(2) |
| ページ | 267-79 |
| 公開日 | 2005-10-24 |
| DOI | 10.1083/jcb.200506124 |
| PII | jcb.200506124 |
| PMID | 16247027 |
| PMC | PMC2171198 |
| MeSH | Anaphase / physiology Animals Aurora Kinase B Caenorhabditis elegans / cytology Caenorhabditis elegans / embryology* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism* Cell Division / physiology* Kinesins / metabolism Protein Serine-Threonine Kinases / metabolism RNA Interference / physiology RNA Nucleotidyltransferases / metabolism* RNA-Binding Proteins / metabolism* Spindle Apparatus / chemistry Spindle Apparatus / metabolism |
| IF | 8.811 |
| 引用数 | 164 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1753 |