| RRC ID |
3389
|
| Author |
Kadandale P, Stewart-Michaelis A, Gordon S, Rubin J, Klancer R, Schweinsberg P, Grant BD, Singson A.
|
| Title |
The egg surface LDL receptor repeat-containing proteins EGG-1 and EGG-2 are required for fertilization in Caenorhabditis elegans.
|
| Journal |
Curr Biol
|
| Abstract |
The molecular machinery that mediates sperm-egg interactions at fertilization is largely unknown. We identify two partially redundant egg surface LDL receptor repeat-containing proteins (EGG-1 and EGG-2) that are required for Caenorhabditis elegans fertility in hermaphrodites, but not males. Wild-type sperm cannot enter the morphologically normal oocytes produced by hermaphrodites that lack egg-1 and egg-2 function despite direct gamete contact. Furthermore, we find that levels of meiotic maturation/ovulation and sperm migratory behavior are altered in egg-1 mutants. These observations suggest an unexpected regulatory link between fertilization and other events necessary for reproductive success. egg-1 and egg-2 are the result of a gene duplication in the nematode lineage leading to C. elegans. The two closely related species C. briggsae and C. remanei encode only a single egg-1/egg-2 homolog that is required for hermaphrodite/female fertility. In addition to being the first identified egg components of the nematode fertilization machinery, the egg-1 and egg-2 gene duplication could be vital with regards to maximizing C. elegans fecundity and understanding the evolutionary differentiation of molecular function and speciation.
|
| Volume |
15(24)
|
| Pages |
2222-9
|
| Published |
2005-12-20
|
| DOI |
10.1016/j.cub.2005.10.043
|
| PII |
S0960-9822(05)01296-0
|
| PMID |
16360684
|
| MeSH |
Animals
Animals, Genetically Modified
Caenorhabditis elegans / physiology*
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Cluster Analysis
Computational Biology
Female
Fertilization / physiology*
Green Fluorescent Proteins
Male
Ovum / metabolism*
Phylogeny*
RNA Interference
Receptors, Cell Surface / metabolism*
Receptors, LDL / metabolism*
Sequence Analysis, DNA
Sperm-Ovum Interactions / physiology*
|
| IF |
9.601
|
| Times Cited |
35
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| C.elegans |
tm1071 |
