論文 - 詳細
| RRC ID | 3390 |
|---|---|
| 著者 | Martin JS, Winkelmann N, Petalcorin MI, McIlwraith MJ, Boulton SJ. |
| タイトル | RAD-51-dependent and -independent roles of a Caenorhabditis elegans BRCA2-related protein during DNA double-strand break repair. |
| ジャーナル | Mol Cell Biol |
| Abstract |
The BRCA2 tumor suppressor is implicated in DNA double-strand break (DSB) repair by homologous recombination (HR), where it regulates the RAD51 recombinase. We describe a BRCA2-related protein of Caenorhabditis elegans (CeBRC-2) that interacts directly with RAD-51 via a single BRC motif and that binds preferentially to single-stranded DNA through an oligonucleotide-oligosaccharide binding fold. Cebrc-2 mutants fail to repair meiotic or radiation-induced DSBs by HR due to inefficient RAD-51 nuclear localization and a failure to target RAD-51 to sites of DSBs. Genetic and cytological comparisons of Cebrc-2 and rad-51 mutants revealed fundamental phenotypic differences that suggest a role for Cebrc-2 in promoting the use of an alternative repair pathway in the absence of rad-51 and independent of nonhomologous end joining (NHEJ). Unlike rad-51 mutants, Cebrc-2 mutants also accumulate RPA-1 at DSBs, and abnormal chromosome aggregates that arise during the meiotic prophase can be rescued by blocking the NHEJ pathway. CeBRC-2 also forms foci in response to DNA damage and can do so independently of rad-51. Thus, CeBRC-2 not only regulates RAD-51 during HR but can also function independently of rad-51 in DSB repair processes. |
| 巻・号 | 25(8) |
| ページ | 3127-39 |
| 公開日 | 2005-4-1 |
| DOI | 10.1128/MCB.25.8.3127-3139.2005 |
| PII | 25/8/3127 |
| PMID | 15798199 |
| PMC | PMC1069622 |
| MeSH | Amino Acid Motifs Amino Acid Sequence Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins DNA / radiation effects DNA Damage* DNA Repair / physiology* DNA-Binding Proteins / analysis DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* DNA-Binding Proteins / physiology* Embryo, Nonmammalian Gene Deletion Genes, Lethal Germ Cells / chemistry Germ Cells / metabolism Germ Cells / radiation effects Molecular Sequence Data Rad51 Recombinase Replication Protein A |
| IF | 3.611 |
| 引用数 | 108 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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| 線虫 | tm1086 |