| RRC ID |
3395
|
| 著者 |
Qin H, Burnette DT, Bae YK, Forscher P, Barr MM, Rosenbaum JL.
|
| タイトル |
Intraflagellar transport is required for the vectorial movement of TRPV channels in the ciliary membrane.
|
| ジャーナル |
Curr Biol
|
| Abstract |
The membranes of all eukaryotic motile (9 + 2) and immotile primary (9 + 0) cilia harbor channels and receptors involved in sensory transduction (reviewed by). These membrane proteins are transported from the cytoplasm onto the ciliary membrane by vesicles targeted for exocytosis at a point adjacent to the ciliary basal body. Here, we use time-lapse fluorescence microscopy to demonstrate that select GFP-tagged sensory receptors undergo rapid vectorial transport along the entire length of the cilia of Caenorhabditis elegans sensory neurons. Transient receptor potential vanilloid (TRPV) channels OSM-9 and OCR-2 move in ciliary membranes at rates comparable to the intraflagellar transport (IFT) machinery located between the membrane and the underlying axonemal microtubules. OSM-9 motility is disrupted in certain IFT mutant backgrounds. Surprisingly, motility of transient receptor potential polycystin (TRPP) channel PKD-2 (polycystic kidney disease-2), a mechano-receptor, was not detected. Our study demonstrates that IFT, previously shown to be necessary for transport of axonemal components, is also involved in the motility of TRPV membrane protein movement along cilia of C. elegans sensory cells.
|
| 巻・号 |
15(18)
|
| ページ |
1695-9
|
| 公開日 |
2005-9-20
|
| DOI |
10.1016/j.cub.2005.08.047
|
| PII |
S0960-9822(05)00966-8
|
| PMID |
16169494
|
| MeSH |
Animals
Biological Transport / physiology
Caenorhabditis elegans / physiology*
Caenorhabditis elegans Proteins / metabolism*
Cell Membrane / metabolism*
Cilia / metabolism*
Green Fluorescent Proteins
Ion Channels / metabolism*
Membrane Proteins / metabolism
Microscopy, Fluorescence
Nerve Tissue Proteins / metabolism*
Neurons, Afferent / metabolism*
Neurons, Afferent / physiology
TRPP Cation Channels
TRPV Cation Channels
Transient Receptor Potential Channels / metabolism*
|
| IF |
9.601
|
| 引用数 |
141
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 線虫 |
tm324 |
