RRC ID 33953
Author Dujeancourt L, Richter R, Chrzanowska-Lightowlers ZM, Bonnefoy N, Herbert CJ.
Title Interactions between peptidyl tRNA hydrolase homologs and the ribosomal release factor Mrf1 in S. pombe mitochondria.
Journal Mitochondrion
Abstract Mitochondrial translation synthesizes key subunits of the respiratory complexes. In Schizosaccharomyces pombe, strains lacking Mrf1, the mitochondrial stop codon recognition factor, are viable, suggesting that other factors can play a role in translation termination. S. pombe contains four predicted peptidyl tRNA hydrolases, two of which (Pth3 and Pth4), have a GGQ motif that is conserved in class I release factors. We show that high dosage of Pth4 can compensate for the absence of Mrf1 and loss of Pth4 exacerbates the lack of Mrf1. Also Pth4 is a component of the mitochondrial ribosome, suggesting that it could help recycling stalled ribosomes.
Volume 13(6)
Pages 871-80
Published 2013-11-1
DOI 10.1016/j.mito.2013.07.115
PII S1567-7249(13)00214-6
PMID 23892058
PMC PMC3919214
MeSH Amino Acid Sequence Carboxylic Ester Hydrolases / metabolism* Gene Deletion Immunoblotting Mitochondria / metabolism* Molecular Sequence Data Schizosaccharomyces / enzymology* Schizosaccharomyces / genetics Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / chemistry Schizosaccharomyces pombe Proteins / metabolism* Sequence Homology, Amino Acid Terminator Regions, Genetic
IF 3.992
Times Cited 7
DNA material pDUAL-YFH1 (RDB06191) pDUAL-FFH1 (RDB06185) S. pombe YFP-FLAG-His clones SpYFH50B06 (SPW100030) SpYFH41F09 (SPW096529) SpYFH25C12 (SPW090060).