RRC ID 34019
著者 Kim BJ, Hong SH, Kook YH, Kim BJ.
タイトル Mycobacterium paragordonae sp. nov., a slowly growing, scotochromogenic species closely related to Mycobacterium gordonae.
ジャーナル Int J Syst Evol Microbiol
Abstract A previously undescribed, slowly growing, scotochromogenic mycobacterial strain (49061(T)) was isolated from a patient with pulmonary infections during the hsp65-sequence-based identification of Korean clinical isolates. Its 16S rRNA gene sequence was unique and the phylogenetic analysis based on 16S rRNA gene sequence (1393 bp) placed the organism into the slow-growing Mycobacterium group close to Mycobacterium gordonae (99.0 % sequence similarity). Growth characteristics and acid-fastness also supported the placement of this species into the genus Mycobacterium. Phenotypically, this strain was generally similar to Mycobacterium gordonae; however, of particular interest, the optimal growth temperature of strain 49061(T) was 25-30 °C, and it was not able to grow at 37 °C on 7H10 agar slants. Unique MALDI-TOF MS profiles of lipids, phylogenetic analysis based on another two gene sequences (hsp65 and rpoB) and a low DNA-DNA relatedness (46.52±0.7) strongly supported the taxonomic status of this strain as a representative of a distinct species from M. gordonae. It was concluded that the strain represents a novel species for which the name Mycobacterium paragordonae is proposed with the type strain 49061(T) ( = JCM 18565(T) = KCTC 29126(T)).
巻・号 64(Pt 1)
ページ 39-45
公開日 2014-1-1
DOI 10.1099/ijs.0.051540-0
PMID 24006480
MeSH Bacterial Typing Techniques Base Sequence DNA, Bacterial / genetics Genes, Bacterial Humans Lung Diseases / microbiology Molecular Sequence Data Mycobacterium / classification* Mycobacterium / genetics Mycobacterium / growth & development Mycobacterium / isolation & purification Mycobacterium Infections / microbiology Nontuberculous Mycobacteria Nucleic Acid Hybridization Phylogeny* RNA, Ribosomal, 16S / genetics Sputum / microbiology
IF 2.415
引用数 22
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 18565