RRC ID 34030
Author Han D, Cui HL.
Title Halosimplex pelagicum sp. nov. and Halosimplex rubrum sp. nov., isolated from salted brown alga Laminaria, and emended description of the genus Halosimplex.
Journal Int. J. Syst. Evol. Microbiol.
Abstract Two halophilic archaeal strains, R2(T) and R27(T), were isolated from the brown alga Laminaria produced at Dalian, Liaoning Province, China. Both had pleomorphic cells that lysed in distilled water, stained Gram-negative and formed red-pigmented colonies. They grew optimally at 42 °C, pH 7.0 and in the presence of 3.1-3.4 M NaCl and 0.03-0.5 M Mg(2+). The major polar lipids of the two strains were phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me) and four major glycolipids chromatographically identical to those of Halosimplex carlsbadense JCM 11222(T). 16S rRNA gene analysis revealed that each strain had two dissimilar 16S rRNA genes and both strains were phylogenetically related to Halosimplex carlsbadense JCM 11222(T) (92.7-98.8 % similarities). The rpoB' gene similarities between strains R2(T) and R27(T) and between these strains and Halosimplex carlsbadense JCM 11222(T) were 95.7 %, 96.1 % and 95.8 %, respectively. The DNA G+C contents of strains R2(T) and R27(T) were 62.5 mol% and 64.0 mol%, respectively. The DNA-DNA hybridization values between strains R2(T) and R27(T) and between the two strains and Halosimplex carlsbadense JCM 11222(T) were 43 %, 52 % and 47 %, respectively. It was concluded that strain R2(T) ( = CGMCC 1.10586(T) = JCM 17263(T)) and strain R27(T) ( = CGMCC 1.10591(T) = JCM 17268(T)) represent two novel species of the genus Halosimplex, for which the names Halosimplex pelagicum sp. nov. and Halosimplex rubrum sp. nov. are proposed. An emended description of the genus Halosimplex is also presented.
Volume 64(Pt 1)
Pages 169-73
Published 2014-1
DOI 10.1099/ijs.0.056887-0
PII ijs.0.056887-0
PMID 24048865
MeSH Base Composition China DNA, Archaeal / genetics Genes, Archaeal Glycolipids / chemistry Halobacteriaceae / classification* Halobacteriaceae / genetics Halobacteriaceae / isolation & purification Laminaria / microbiology* Molecular Sequence Data Nucleic Acid Hybridization Phylogeny* Pigmentation RNA, Ribosomal, 16S / genetics Salts Sequence Analysis, DNA
IF 1.932
Times Cited 7
WOS Category MICROBIOLOGY
Resource
General Microbes JCM 9738 JCM 11222 JCM 17263 JCM 17268