論文 - 詳細
| RRC ID | 34047 |
|---|---|
| 著者 | Schirmack J, Mangelsdorf K, Ganzert L, Sand W, Hillebrand-Voiculescu A, Wagner D. |
| タイトル | Methanobacterium movilense sp. nov., a hydrogenotrophic, secondary-alcohol-utilizing methanogen from the anoxic sediment of a subsurface lake. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
A novel strain of methanogenic archaea, designated MC-20(T), was isolated from the anoxic sediment of a subsurface lake in Movile Cave, Mangalia, Romania. Cells were non-motile, Gram-stain-negative rods 3.5-4.0 µm in length and 0.6-0.7 µm in width, and occurred either singly or in short chains. Strain MC-20(T) was able to utilize H2/CO2, formate, 2-propanol and 2-butanol as substrate, but not acetate, methanol, ethanol, dimethyl sulfide, monomethylamine, dimethylamine or trimethylamine. Neither trypticase peptone nor yeast extract was required for growth. The major membrane lipids of strain MC-20(T) were archaeol phosphatidylethanolamine and diglycosyl archaeol, while archaeol phosphatidylinositol and glycosyl archaeol were present only in minor amounts. Optimal growth was observed at 33 °C, pH 7.4 and 0.08 M NaCl. Based on phylogenetic analysis of 16S rRNA gene sequences, strain MC-20(T) was closely affiliated with Methanobacterium oryzae FPi(T) (similarity 97.1%) and Methanobacterium lacus 17A1(T) (97.0%). The G+C content of the genomic DNA was 33.0 mol%. Based on phenotypic and genotypic differences, strain MC-20(T) was assigned to a novel species of the genus Methanobacterium for which the name Methanobacterium movilense sp. nov. is proposed. The type strain is MC-20(T) ( = DSM 26032(T) = JCM 18470(T)). |
| 巻・号 | 64(Pt 2) |
| ページ | 522-527 |
| 公開日 | 2014-2-1 |
| DOI | 10.1099/ijs.0.057224-0 |
| PMID | 24108325 |
| MeSH | Base Composition Caves / microbiology* DNA, Archaeal / genetics Geologic Sediments / microbiology* Lakes / microbiology Methanobacterium / classification* Methanobacterium / genetics Methanobacterium / isolation & purification Molecular Sequence Data Phylogeny* RNA, Ribosomal, 16S / genetics Romania Sequence Analysis, DNA |
| IF | 2.415 |
| 引用数 | 25 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 18470 |