RRC ID 34068
著者 Zhou L, Liu X, Dong X.
タイトル Methanospirillum psychrodurum sp. nov., isolated from wetland soil.
ジャーナル Int J Syst Evol Microbiol
Abstract A psychrotolerant methanogenic strain, X-18(T), was isolated from the soil of the Madoi wetland at Qinghai, Tibetan plateau, China. Cells were wavy rods (11-62 µm long) with blunt tapered ends and Gram-stain-negative. Strain X-18(T) grew strictly anaerobically and produced methane exclusively from H2/CO2. Growth occurred in the temperature range of 4-32 °C and optimally at 25 °C. Growth pH ranged from 6.5 to 8.0 and the optimum was 7.0. The G+C content of the genomic DNA of strain X-18(T) was 44.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences and the alpha subunit of methyl-coenzyme M reductase indicated that strain X-18(T) was affiliated to the genus Methanospirillum and was most closely related to Methanospirillum lacunae Ki8-1(T), with 96.3% 16S rRNA gene sequence similarity. However, strain X-18(T) could be distinguished from the existing species of the genus Methanospirillum by its lower growth temperature and obligate hydrogenotrophic methanogenesis. On the basis of phenotypic characteristics and phylogenetic analysis, strain X-18(T) represents a novel species of the genus Methanospirillum, for which the name Methanospirillum psychrodurum sp. nov. is proposed and strain X-18(T) is assigned as the type strain ( = CGMCC 1.5186(T) = JCM 19216(T)).
巻・号 64(Pt 2)
ページ 638-641
公開日 2014-2-1
DOI 10.1099/ijs.0.057299-0
PMID 24158951
MeSH Base Composition China DNA, Archaeal / genetics Methane / biosynthesis Methanospirillum / classification* Methanospirillum / genetics Methanospirillum / isolation & purification Molecular Sequence Data Oxidoreductases / genetics Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Soil Microbiology* Wetlands*
IF 2.415
引用数 9
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 10133 JCM 19216