論文 - 詳細
| RRC ID | 34068 |
|---|---|
| 著者 | Zhou L, Liu X, Dong X. |
| タイトル | Methanospirillum psychrodurum sp. nov., isolated from wetland soil. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
A psychrotolerant methanogenic strain, X-18(T), was isolated from the soil of the Madoi wetland at Qinghai, Tibetan plateau, China. Cells were wavy rods (11-62 µm long) with blunt tapered ends and Gram-stain-negative. Strain X-18(T) grew strictly anaerobically and produced methane exclusively from H2/CO2. Growth occurred in the temperature range of 4-32 °C and optimally at 25 °C. Growth pH ranged from 6.5 to 8.0 and the optimum was 7.0. The G+C content of the genomic DNA of strain X-18(T) was 44.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences and the alpha subunit of methyl-coenzyme M reductase indicated that strain X-18(T) was affiliated to the genus Methanospirillum and was most closely related to Methanospirillum lacunae Ki8-1(T), with 96.3% 16S rRNA gene sequence similarity. However, strain X-18(T) could be distinguished from the existing species of the genus Methanospirillum by its lower growth temperature and obligate hydrogenotrophic methanogenesis. On the basis of phenotypic characteristics and phylogenetic analysis, strain X-18(T) represents a novel species of the genus Methanospirillum, for which the name Methanospirillum psychrodurum sp. nov. is proposed and strain X-18(T) is assigned as the type strain ( = CGMCC 1.5186(T) = JCM 19216(T)). |
| 巻・号 | 64(Pt 2) |
| ページ | 638-641 |
| 公開日 | 2014-2-1 |
| DOI | 10.1099/ijs.0.057299-0 |
| PMID | 24158951 |
| MeSH | Base Composition China DNA, Archaeal / genetics Methane / biosynthesis Methanospirillum / classification* Methanospirillum / genetics Methanospirillum / isolation & purification Molecular Sequence Data Oxidoreductases / genetics Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Soil Microbiology* Wetlands* |
| IF | 2.415 |
| 引用数 | 9 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 10133 JCM 19216 |