RRC ID |
34073
|
著者 |
Bello-López JM, Navarro-Noya YE, Gómez-Acata S, Hernández-Montañez Z, Dendooven L.
|
タイトル |
Identification of α-amylase by random and specific mutagenesis of Texcoconibacillus texcoconensis 13CCT strain isolated from extreme alkaline-saline soil of the former Lake Texcoco (Mexico).
|
ジャーナル |
Folia Microbiol (Praha)
|
Abstract |
The alkaline α-amylase produced by Texcoconibacillus texcoconensis 13CC(T) strain was identified by random mutagenesis and confirmed by directed mutagenesis. A transposon mutagenesis approach was taken to identify the gene responsible for the degradation of starch in T. texcoconensis 13CC(T) strain. The deduced amino acids of the amy gene had a 99% similarity with those of Bacillus selenitireducens MLS10 and 97% with those of Paenibacillus curdlanolyticus YK9. The enzyme showed a maximum activity of 131.1 U/mL at 37 °C and pH 9.5 to 10.5. In situ activity of the enzyme determined by polyacrylamide gel electrophoresis showed only one band with amylolytic activity. This is the first report of a bacterium isolated from the extreme alkaline-saline soil of the former Lake Texcoco (Mexico) with amylolytic activity in alkaline conditions while its potential as a source of amylases for the industry is discussed.
|
巻・号 |
59(3)
|
ページ |
235-40
|
公開日 |
2014-5-1
|
DOI |
10.1007/s12223-013-0289-8
|
PMID |
24186808
|
MeSH |
Bacillus / enzymology*
Bacillus / genetics*
Bacillus / growth & development
Bacillus / isolation & purification
Lakes
Mexico
Mutagenesis*
Mutation
Salinity*
Soil / chemistry*
Soil Microbiology*
alpha-Amylases / genetics
alpha-Amylases / metabolism*
|
IF |
1.73
|
引用数 |
0
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
|
リソース情報 |
一般微生物 |
JCM 17654 |