論文 - 詳細
| RRC ID | 34073 |
|---|---|
| 著者 | Bello-López JM, Navarro-Noya YE, Gómez-Acata S, Hernández-Montañez Z, Dendooven L. |
| タイトル | Identification of α-amylase by random and specific mutagenesis of Texcoconibacillus texcoconensis 13CCT strain isolated from extreme alkaline-saline soil of the former Lake Texcoco (Mexico). |
| ジャーナル | Folia Microbiol (Praha) |
| Abstract |
The alkaline α-amylase produced by Texcoconibacillus texcoconensis 13CC(T) strain was identified by random mutagenesis and confirmed by directed mutagenesis. A transposon mutagenesis approach was taken to identify the gene responsible for the degradation of starch in T. texcoconensis 13CC(T) strain. The deduced amino acids of the amy gene had a 99% similarity with those of Bacillus selenitireducens MLS10 and 97% with those of Paenibacillus curdlanolyticus YK9. The enzyme showed a maximum activity of 131.1 U/mL at 37 °C and pH 9.5 to 10.5. In situ activity of the enzyme determined by polyacrylamide gel electrophoresis showed only one band with amylolytic activity. This is the first report of a bacterium isolated from the extreme alkaline-saline soil of the former Lake Texcoco (Mexico) with amylolytic activity in alkaline conditions while its potential as a source of amylases for the industry is discussed. |
| 巻・号 | 59(3) |
| ページ | 235-40 |
| 公開日 | 2014-5-1 |
| DOI | 10.1007/s12223-013-0289-8 |
| PMID | 24186808 |
| MeSH | Bacillus / enzymology* Bacillus / genetics* Bacillus / growth & development Bacillus / isolation & purification Lakes Mexico Mutagenesis* Mutation Salinity* Soil / chemistry* Soil Microbiology* alpha-Amylases / genetics alpha-Amylases / metabolism* |
| IF | 1.73 |
| 引用数 | 0 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 17654 |