RRC ID 34073
著者 Bello-López JM, Navarro-Noya YE, Gómez-Acata S, Hernández-Montañez Z, Dendooven L.
タイトル Identification of α-amylase by random and specific mutagenesis of Texcoconibacillus texcoconensis 13CCT strain isolated from extreme alkaline-saline soil of the former Lake Texcoco (Mexico).
ジャーナル Folia Microbiol (Praha)
Abstract The alkaline α-amylase produced by Texcoconibacillus texcoconensis 13CC(T) strain was identified by random mutagenesis and confirmed by directed mutagenesis. A transposon mutagenesis approach was taken to identify the gene responsible for the degradation of starch in T. texcoconensis 13CC(T) strain. The deduced amino acids of the amy gene had a 99% similarity with those of Bacillus selenitireducens MLS10 and 97% with those of Paenibacillus curdlanolyticus YK9. The enzyme showed a maximum activity of 131.1 U/mL at 37 °C and pH 9.5 to 10.5. In situ activity of the enzyme determined by polyacrylamide gel electrophoresis showed only one band with amylolytic activity. This is the first report of a bacterium isolated from the extreme alkaline-saline soil of the former Lake Texcoco (Mexico) with amylolytic activity in alkaline conditions while its potential as a source of amylases for the industry is discussed.
巻・号 59(3)
ページ 235-40
公開日 2014-5-1
DOI 10.1007/s12223-013-0289-8
PMID 24186808
MeSH Bacillus / enzymology* Bacillus / genetics* Bacillus / growth & development Bacillus / isolation & purification Lakes Mexico Mutagenesis* Mutation Salinity* Soil / chemistry* Soil Microbiology* alpha-Amylases / genetics alpha-Amylases / metabolism*
IF 1.73
引用数 0
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
一般微生物 JCM 17654