RRC ID 3409
著者 Blacque OE, Li C, Inglis PN, Esmail MA, Ou G, Mah AK, Baillie DL, Scholey JM, Leroux MR.
タイトル The WD repeat-containing protein IFTA-1 is required for retrograde intraflagellar transport.
ジャーナル Mol Biol Cell
Abstract The assembly and maintenance of cilia require intraflagellar transport (IFT), a microtubule-dependent bidirectional motility of multisubunit protein complexes along ciliary axonemes. Defects in IFT and the functions of motile or sensory cilia are associated with numerous human ailments, including polycystic kidney disease and Bardet-Biedl syndrome. Here, we identify a novel Caenorhabditis elegans IFT gene, IFT-associated gene 1 (ifta-1), which encodes a WD repeat-containing protein with strong homology to a mammalian protein of unknown function. Both the C. elegans and human IFTA-1 proteins localize to the base of cilia, and in C. elegans, IFTA-1 can be observed to undergo IFT. IFTA-1 is required for the function and assembly of cilia, because a C. elegans ifta-1 mutant displays chemosensory abnormalities and shortened cilia with prominent ciliary accumulations of core IFT machinery components that are indicative of retrograde transport defects. Analyses of C. elegans IFTA-1 localization/motility along bbs mutant cilia, where anterograde IFT assemblies are destabilized, and in a che-11 IFT gene mutant, demonstrate that IFTA-1 is closely associated with the IFT particle A subcomplex, which is implicated in retrograde IFT. Together, our data indicate that IFTA-1 is a novel IFT protein that is required for retrograde transport along ciliary axonemes.
巻・号 17(12)
ページ 5053-62
公開日 2006-12-1
DOI 10.1091/mbc.e06-06-0571
PII E06-06-0571
PMID 17021254
PMC PMC1679672
MeSH Animals Base Sequence Biological Transport Caenorhabditis elegans / cytology Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cilia / metabolism Flagella / metabolism* Humans Models, Biological Molecular Sequence Data Multiprotein Complexes / metabolism Mutation / genetics Protein Transport Repetitive Sequences, Amino Acid*
IF 3.791
引用数 70
WOS 分野 CELL BIOLOGY
リソース情報
線虫 tm324