RRC ID 34096
著者 Singh P, Singh SM, Dhakephalkar P.
タイトル Diversity, cold active enzymes and adaptation strategies of bacteria inhabiting glacier cryoconite holes of High Arctic.
ジャーナル Extremophiles
Abstract Cryoconite holes have biogeochemical, ecological and biotechnological importance. This communication presents results on culturable psychrophilic bacterial diversity from cryoconite holes at Midre Lovénbreen (ML), Austre Brøggerbreen (AB), and Vestre Brøggerbreen (VB) glaciers. The culturable bacterial count ranged from 2.7 × 10(3) to 8.8 × 10(4) CFUs/g while the total bacterial numbers ranged from 5.07 × 10(5) to 1.50 × 10(6) cells at the three glaciers. A total of 35 morphologically distinct bacterial isolates were isolated. Based on 16S rRNA gene sequence data, the identified species belonged to eight genera namely Pseudomonas, Polaromonas, Micrococcus, Subtercola, Agreia, Leifsonia, Cryobacterium and Flavobacterium. The isolates varied in their growth temperature, NaCl tolerance, growth pH, enzyme activities, carbon utilization and antibiotic sensitivity tests. Fatty acid profiles indicate the predominance of branched fatty acids in the isolates. To the best of our knowledge, this is the first record of culturable bacterial communities and their characterization from glacier cryoconites from High Arctic. High amylase and protease activities expressed by Micrococcus sp. MLB-41 and amylase, protease and lipase activities expressed by Cryobacterium sp. MLB-32 provide a clue to the potential applications of these organisms. These cold-adapted enzymes may provide an opportunity for the prospect of biotechnology in Arctic.
巻・号 18(2)
ページ 229-42
公開日 2014-3-1
DOI 10.1007/s00792-013-0609-6
PMID 24346230
MeSH Adaptation, Physiological* Arctic Regions Bacteria / isolation & purification* Bacterial Proteins / genetics Bacterial Proteins / metabolism Enzymes / genetics Enzymes / metabolism Ice Cover / microbiology* Microbiota* Phenotype
IF 2.462
引用数 36
WOS 分野 MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
一般微生物 JCM 19504 JCM 19508