論文 - 詳細
| RRC ID | 34190 |
|---|---|
| 著者 | Dong L, Ming H, Yin YR, Duan YY, Zhou EM, Nie GX, Feng HG, Liu L, Li WJ. |
| タイトル | Roseomonas alkaliterrae sp. nov., isolated from an alkali geothermal soil sample in Tengchong, Yunnan, South-West China. |
| ジャーナル | Antonie Van Leeuwenhoek |
| Abstract |
An alkalitolerant, thermotolerant and Gram-stain negative bacterium, designated strain YIM 78007(T), was isolated from an alkaline geothermal soil sample from Hehua hot spring, Tengchong, Yunnan province, south-west China. Cells of strain YIM 78007(T) were observed to be aerobic and short rod-shaped. The colonies were observed to be orange-red, convex and circular. 16S rRNA gene sequence-based phylogenetic analysis showed that strain YIM 78007(T) clustered with members of the genus Roseomonas (with similarities from 97.2 to 92.2 %). Optimal growth of strain YIM 78007 occurs at 40-50 °C and pH 8.0-10.0. The predominant ubiquinone was identified as Q-10 and the major fatty acids were identified as C18:1 ω7c and C16:0. The polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, two unidentified aminolipids and one unknown phospholipid. The G + C content of the genomic DNA was determined to be 63 mol %. The levels of DNA-DNA hybridization relatedness between strain YIM 78007(T) and its closet neighbours (Roseomonas lacus JCM 13283(T) and Roseomonas terrae JCM 14592(T)) were well below the threshold required for the proposal of a novel species. The results of physiological and biochemical characteristics, the phylogenetic analysis, as well as low DNA-DNA hybridization values, allowed the phenotypic and genotypic differentiation of strain YIM 78007(T) from its closest phylogenetic neighbours. Therefore, strain YIM 78007(T) is considered to represent a novel species of the genus Roseomonas, for which the name Roseomonas alkaliterrae sp. nov. is proposed. The type strain is YIM 78007(T) (=BCRC 80644(T) = JCM 19656(T)). |
| 巻・号 | 105(5) |
| ページ | 899-905 |
| 公開日 | 2014-5-1 |
| DOI | 10.1007/s10482-014-0144-1 |
| PMID | 24590559 |
| MeSH | Bacterial Typing Techniques Base Composition China Cluster Analysis DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Fatty Acids / analysis Hot Springs Methylobacteriaceae / classification* Methylobacteriaceae / genetics Methylobacteriaceae / isolation & purification* Methylobacteriaceae / physiology Molecular Sequence Data Nucleic Acid Hybridization Phospholipids / analysis Phylogeny Quinones / analysis RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Soil Microbiology* |
| IF | 2.674 |
| 引用数 | 15 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 13283 JCM 14592 JCM 19656 |