論文 - 詳細
| RRC ID | 34197 |
|---|---|
| 著者 | L'Haridon S, Chalopin M, Colombo D, Toffin L. |
| タイトル | Methanococcoides vulcani sp. nov., a marine methylotrophic methanogen that uses betaine, choline and N,N-dimethylethanolamine for methanogenesis, isolated from a mud volcano, and emended description of the genus Methanococcoides. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
A novel, strictly anaerobic, methylotrophic marine methanogen, strain SLH33(T), was isolated from deep sediment samples covered by an orange microbial mat collected from the Napoli Mud Volcano. Cells of strain SLH33(T) were Gram-stain-negative, motile, irregular cocci that occurred singly. Cells utilized trimethylamine, dimethylamine, monomethylamine, methanol, betaine, N,N-dimethylethanolamine and choline (N,N,N-trimethylethanolamine) as substrates for growth and methanogenesis. The optimal growth temperature was 30 °C; maximum growth rate was obtained at pH 7.0 in the presence of 0.5 M Na(+). The DNA G+C content of strain SLH33(T) was 43.4 mol%. Phylogenetic analyses based on 16S rRNA gene sequences placed strain SLH33(T) within the genus Methanococcoides. The novel isolate was related most closely to Methanococcoides methylutens TMA-10(T) (98.8% 16S rRNA gene sequence similarity) but distantly related to Methanococcoides burtonii DSM 6242(T) (97.6%) and Methanococcoides alaskense AK-5(T) (97.6%). DNA-DNA hybridization studies indicated that strain SLH33(T) represents a novel species, given that it shared less than 16% DNA-DNA relatedness with Methanococcoides methylutens TMA-10(T). The name Methanococcoides vulcani sp. nov. is proposed for this novel species, with strain SLH33(T) ( = DSM 26966(T) = JCM 19278(T)) as the type strain. An emended description of the genus Methanococcoides is also proposed. |
| 巻・号 | 64(Pt 6) |
| ページ | 1978-1983 |
| 公開日 | 2014-6-1 |
| DOI | 10.1099/ijs.0.058289-0 |
| PMID | 24614846 |
| MeSH | Base Composition Betaine / metabolism Choline / metabolism DNA, Bacterial / genetics Deanol / metabolism Hydrothermal Vents / microbiology* Mediterranean Sea Methanosarcinaceae / classification* Methanosarcinaceae / genetics Methanosarcinaceae / isolation & purification Molecular Sequence Data Nucleic Acid Hybridization Phylogeny* Pigmentation RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA |
| IF | 2.415 |
| 引用数 | 18 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 19278 |