RRC ID 34197
Author L'Haridon S, Chalopin M, Colombo D, Toffin L.
Title Methanococcoides vulcani sp. nov., a marine methylotrophic methanogen that uses betaine, choline and N,N-dimethylethanolamine for methanogenesis, isolated from a mud volcano, and emended description of the genus Methanococcoides.
Journal Int. J. Syst. Evol. Microbiol.
Abstract A novel, strictly anaerobic, methylotrophic marine methanogen, strain SLH33(T), was isolated from deep sediment samples covered by an orange microbial mat collected from the Napoli Mud Volcano. Cells of strain SLH33(T) were Gram-stain-negative, motile, irregular cocci that occurred singly. Cells utilized trimethylamine, dimethylamine, monomethylamine, methanol, betaine, N,N-dimethylethanolamine and choline (N,N,N-trimethylethanolamine) as substrates for growth and methanogenesis. The optimal growth temperature was 30 °C; maximum growth rate was obtained at pH 7.0 in the presence of 0.5 M Na(+). The DNA G+C content of strain SLH33(T) was 43.4 mol%. Phylogenetic analyses based on 16S rRNA gene sequences placed strain SLH33(T) within the genus Methanococcoides. The novel isolate was related most closely to Methanococcoides methylutens TMA-10(T) (98.8% 16S rRNA gene sequence similarity) but distantly related to Methanococcoides burtonii DSM 6242(T) (97.6%) and Methanococcoides alaskense AK-5(T) (97.6%). DNA-DNA hybridization studies indicated that strain SLH33(T) represents a novel species, given that it shared less than 16% DNA-DNA relatedness with Methanococcoides methylutens TMA-10(T). The name Methanococcoides vulcani sp. nov. is proposed for this novel species, with strain SLH33(T) ( = DSM 26966(T) = JCM 19278(T)) as the type strain. An emended description of the genus Methanococcoides is also proposed.
Volume 64(Pt 6)
Pages 1978-83
Published 2014-6
DOI 10.1099/ijs.0.058289-0
PII ijs.0.058289-0
PMID 24614846
MeSH Base Composition Betaine / metabolism Choline / metabolism DNA, Bacterial / genetics Deanol / metabolism Hydrothermal Vents / microbiology* Mediterranean Sea Methanosarcinaceae / classification* Methanosarcinaceae / genetics Methanosarcinaceae / isolation & purification Molecular Sequence Data Nucleic Acid Hybridization Phylogeny* Pigmentation RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA
IF 2.134
Times Cited 6
WOS Category MICROBIOLOGY
Resource
General Microbes JCM 19278