論文 - 詳細
| RRC ID | 34209 |
|---|---|
| 著者 | Takei S, Omoto C, Kitagawa K, Morishita N, Katayama T, Shigemura K, Fujisawa M, Kawabata M, Hotta H, Shirakawa T. |
| タイトル | Oral administration of genetically modified Bifidobacterium displaying HCV-NS3 multi-epitope fusion protein could induce an HCV-NS3-specific systemic immune response in mice. |
| ジャーナル | Vaccine |
| Abstract |
More than 170 million people worldwide are chronic HCV (Hepatitis C virus) carriers, and about 30% of them will develop progressive liver disease, such as cirrhosis and hepatocellular carcinoma. A combination of pegylated interferon-α with ribavirin, the standard treatment for HCV infection, has been effective in fewer than 50% of patients infected with HCV genotype 1. A strong T cell response against the nonstructural protein 3 (NS3) is important for recovery from acute HCV infection, and an early multi-specific CD4+ helper and CD8+ cytotoxic T cell response is critical for HCV clearance. In the present study, we successfully constructed a genetically modified Bifidobacterium longum (B. longum) displaying recombinant HCV-NS3 peptides containing some CD4 and CD8 epitopes located in the HCV-NS3 region as an oral vaccine against chronic HCV infection. The oral administration of this vaccine could induce NS3-specific immune responses in mice through intestinal mucosal immunity. Our findings suggest that this novel oral vaccine has great potential as a novel oral vaccine against chronic HCV infection. |
| 巻・号 | 32(25) |
| ページ | 3066-74 |
| 公開日 | 2014-5-23 |
| DOI | 10.1016/j.vaccine.2014.03.022 |
| PII | S0264-410X(14)00366-1 |
| PMID | 24657718 |
| MeSH | Administration, Oral Animals Antibodies, Viral / immunology Bifidobacterium* / genetics Bifidobacterium* / immunology Cytokines / immunology Epitopes / immunology* Female Hepatitis C / prevention & control* Immunity, Mucosal Intestines / immunology Mice, Inbred BALB C Recombinant Fusion Proteins / immunology Spleen / immunology T-Lymphocytes / immunology Viral Hepatitis Vaccines / immunology* Viral Nonstructural Proteins / immunology* |
| IF | 3.143 |
| 引用数 | 16 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 1217 |