Reference - Detail
| RRC ID | 34278 |
|---|---|
| Author | Wang KC, Lyu SY, Liu YC, Chang CY, Wu CJ, Li TL. |
| Title | Insights into the binding specificity and catalytic mechanism of N-acetylhexosamine 1-phosphate kinases through multiple reaction complexes. |
| Journal | Acta Crystallogr D Biol Crystallogr |
| Abstract |
Utilization of N-acetylhexosamine in bifidobacteria requires the specific lacto-N-biose/galacto-N-biose pathway, a pathway differing from the Leloir pathway while establishing symbiosis between humans and bifidobacteria. The gene lnpB in the pathway encodes a novel hexosamine kinase NahK, which catalyzes the formation of N-acetylhexosamine 1-phosphate (GlcNAc-1P/GalNAc-1P). In this report, seven three-dimensional structures of NahK in complex with GlcNAc, GalNAc, GlcNAc-1P, GlcNAc/AMPPNP and GlcNAc-1P/ADP from both Bifidobacterium longum (JCM1217) and B. infantis (ATCC15697) were solved at resolutions of 1.5-2.2 Å. NahK is a monomer in solution, and its polypeptide folds in a crescent-like architecture subdivided into two domains by a deep cleft. The NahK structures presented here represent the first multiple reaction complexes of the enzyme. This structural information reveals the molecular basis for the recognition of the given substrates and products, GlcNAc/GalNAc, GlcNAc-1P/GalNAc-1P, ATP/ADP and Mg(2+), and provides insights into the catalytic mechanism, enabling NahK and mutants thereof to form a choice of biocatalysts for enzymatic and chemoenzymatic synthesis of carbohydrates. |
| Volume | 70(Pt 5) |
| Pages | 1401-10 |
| Published | 2014-5-1 |
| DOI | 10.1107/S1399004714004209 |
| PII | S1399004714004209 |
| PMID | 24816108 |
| MeSH | Acetylglucosamine / metabolism Adenosine Diphosphate / metabolism Adenosine Triphosphate / metabolism Bifidobacterium / enzymology* Bifidobacterium / metabolism Binding Sites Crystallography, X-Ray Magnesium / metabolism Models, Molecular Mutagenesis, Site-Directed Phosphotransferases / chemistry* Phosphotransferases / genetics Phosphotransferases / metabolism* Protein Conformation Substrate Specificity |
| IF | 5.266 |
| Times Cited | 8 |
| WOS Category | CRYSTALLOGRAPHY BIOCHEMICAL RESEARCH METHODS BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | X(Twitter) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM 1217 |