| RRC ID |
34331
|
| 著者 |
Woo PCY, Wu AKL, Tsang CC, Leung KW, Ngan AHY, Curreem SOT, Lam KW, Chen JHK, Chan JFW, Lau SKP.
|
| タイトル |
Streptobacillus hongkongensis sp. nov., isolated from patients with quinsy and septic arthritis, and emended descriptions of the genus Streptobacillus and Streptobacillus moniliformis.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
Two bacterial strains, HKU33(T) and HKU34, were isolated in Hong Kong from the pus aspirated from the right peritonsillar abscess of a patient with quinsy and the left elbow joint fluid of another patient with tophaceous gout and left elbow septic arthritis, respectively. The bacteria were Gram-stain-negative, non-motile, non-spore-forming, non-haemolytic pleomorphic bacilli. They grew best on Columbia agar with 5 % defibrinated sheep blood in an anaerobic environment or aerobic environment with 5 % CO2. They also grew on chocolate agar but not on MacConkey agar. They were catalase- and cytochrome oxidase-negative. They showed a unique profile of enzyme activities distinguishable from their closely related species. Phylogenetic analysis of the complete 16S rRNA gene, and partial groEL, gyrB and recA gene sequences showed the two isolates formed a distinct branch within the family Leptotrichiaceae, being related most closely to Streptobacillus moniliformis. Hierarchical cluster analysis of mass spectra of whole-cell protein contents showed that strains HKU33(T) and HKU34 were closely related to each other, but were distinct from Streptobacillus moniliformis, Sneathia sanguinegens and 'Leptotrichia amnionii'. The DNA G+C content of strain HKU33(T) was 26.0±2.1 mol% (mean±sd; n = 3). DNA-DNA hybridization demonstrated ≤45.02 % DNA relatedness between the two isolates and Streptobacillus moniliformis CCUG 13453(T). A novel species, Streptobacillus hongkongensis sp. nov., is proposed to accommodate strains HKU33(T) and HKU34, with HKU33(T) ( = JCM 18691(T) = NCTC 13659(T) = DSM 26322(T)) designated the type strain. Emended descriptions of the genus Streptobacillus and Streptobacillus moniliformis are also given.
|
| 巻・号 |
64(Pt 9)
|
| ページ |
3034-3039
|
| 公開日 |
2014-9-1
|
| DOI |
10.1099/ijs.0.061242-0
|
| PMID |
24912824
|
| MeSH |
Adult
Arthritis, Infectious / microbiology*
Bacterial Typing Techniques
Base Composition
DNA, Bacterial / genetics
Genes, Bacterial
Hong Kong
Humans
Male
Middle Aged
Molecular Sequence Data
Nucleic Acid Hybridization
Peritonsillar Abscess / microbiology*
Phylogeny*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Streptobacillus / classification*
Streptobacillus / genetics
Streptobacillus / isolation & purification
|
| IF |
2.415
|
| 引用数 |
20
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 18691 |
