RRC ID 34410
Author Ishibashi N, Himeno K, Masuda Y, Perez RH, Iwatani S, Zendo T, Wilaipun P, Leelawatcharamas V, Nakayama J, Sonomoto K.
Title Gene cluster responsible for secretion of and immunity to multiple bacteriocins, the NKR-5-3 enterocins.
Journal Appl. Environ. Microbiol.
Abstract Enterococcus faecium NKR-5-3, isolated from Thai fermented fish, is characterized by the unique ability to produce five bacteriocins, namely, enterocins NKR-5-3A, -B, -C, -D, and -Z (Ent53A, Ent53B, Ent53C, Ent53D, and Ent53Z). Genetic analysis with a genome library revealed that the bacteriocin structural genes (enkA [ent53A], enkC [ent53C], enkD [ent53D], and enkZ [ent53Z]) that encode these peptides (except for Ent53B) are located in close proximity to each other. This NKR-5-3ACDZ (Ent53ACDZ) enterocin gene cluster (approximately 13 kb long) includes certain bacteriocin biosynthetic genes such as an ABC transporter gene (enkT), two immunity genes (enkIaz and enkIc), a response regulator (enkR), and a histidine protein kinase (enkK). Heterologous-expression studies of enkT and ΔenkT mutant strains showed that enkT is responsible for the secretion of Ent53A, Ent53C, Ent53D, and Ent53Z, suggesting that EnkT is a wide-range ABC transporter that contributes to the effective production of these bacteriocins. In addition, EnkIaz and EnkIc were found to confer self-immunity to the respective bacteriocins. Furthermore, bacteriocin induction assays performed with the ΔenkRK mutant strain showed that EnkR and EnkK are regulatory proteins responsible for bacteriocin production and that, together with Ent53D, they constitute a three-component regulatory system. Thus, the Ent53ACDZ gene cluster is essential for the biosynthesis and regulation of NKR-5-3 enterocins, and this is, to our knowledge, the first report that demonstrates the secretion of multiple bacteriocins by an ABC transporter.
Volume 80(21)
Pages 6647-55
Published 2014-11
DOI 10.1128/AEM.02312-14
PII AEM.02312-14
PMID 25149515
PMC PMC4249031
MeSH Anti-Bacterial Agents / pharmacology* Biosynthetic Pathways Bridged-Ring Compounds / pharmacology Drug Resistance, Bacterial* Enterococcus faecium / drug effects* Enterococcus faecium / genetics* Enterococcus faecium / isolation & purification Food Microbiology Gene Expression Regulation, Bacterial Multigene Family* Thailand
IF 3.633
Times Cited 4
WOS Category BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
Resource
General Microbes JCM 1465 JCM 5803 JCM 5804