論文 - 詳細
| RRC ID | 34463 |
|---|---|
| 著者 | Ujihara T, Nagano M, Wada H, Mitsuhashi S. |
| タイトル | Identification of a novel type of polyunsaturated fatty acid synthase involved in arachidonic acid biosynthesis. |
| ジャーナル | FEBS Lett |
| Abstract |
Arachidonic acid (ARA) is a polyunsaturated fatty acid (PUFA) and an essential component of membrane lipids. However, the PUFA synthase required for ARA biosynthesis has not been identified in any organism. To identify the PUFA synthase producing ARA, we determined the draft genome sequence of the marine bacterium Aureispira marina, which produces a high level of ARA, and found a gene cluster encoding a putative PUFA synthase for ARA production. Expression of the gene cluster in Escherichia coli induced production of ARA, demonstrating that the gene cluster encodes a PUFA synthase required for ARA biosynthesis. |
| 巻・号 | 588(21) |
| ページ | 4032-6 |
| 公開日 | 2014-11-3 |
| DOI | 10.1016/j.febslet.2014.09.023 |
| PII | S0014-5793(14)00700-5 |
| PMID | 25263707 |
| MeSH | Arachidonic Acid / biosynthesis* Bacteroidetes / enzymology Bacteroidetes / genetics Bacteroidetes / metabolism Escherichia coli / genetics Fatty Acid Synthases / genetics* Fatty Acid Synthases / metabolism* Multigene Family |
| IF | 3.057 |
| 引用数 | 9 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| 一般微生物 | JCM 23201 |