論文 - 詳細
| RRC ID | 34495 |
|---|---|
| 著者 | Fukuda M, Takeda H, Kato HE, Doki S, Ito K, Maturana AD, Ishitani R, Nureki O. |
| タイトル | Structural basis for dynamic mechanism of nitrate/nitrite antiport by NarK. |
| ジャーナル | Nat Commun |
| Abstract |
NarK belongs to the nitrate/nitrite porter (NNP) family in the major facilitator superfamily (MFS) and plays a central role in nitrate uptake across the membrane in diverse organisms, including archaea, bacteria, fungi and plants. Although previous studies provided insight into the overall structure and the substrate recognition of NarK, its molecular mechanism, including the driving force for nitrate transport, remained elusive. Here we demonstrate that NarK is a nitrate/nitrite antiporter, using an in vitro reconstituted system. Furthermore, we present the high-resolution crystal structures of NarK from Escherichia coli in the nitrate-bound occluded, nitrate-bound inward-open and apo inward-open states. The integrated structural, functional and computational analyses reveal the nitrate/nitrite antiport mechanism of NarK, in which substrate recognition is coupled to the transport cycle by the concomitant movement of the transmembrane helices and the key tyrosine and arginine residues in the substrate-binding site. |
| 巻・号 | 6 |
| ページ | 7097 |
| 公開日 | 2015-5-11 |
| DOI | 10.1038/ncomms8097 |
| PII | ncomms8097 |
| PMID | 25959928 |
| PMC | PMC4432589 |
| MeSH | Anion Transport Proteins / genetics Anion Transport Proteins / metabolism* Binding Sites Cloning, Molecular Escherichia coli / genetics Escherichia coli / metabolism* Gene Expression Regulation, Bacterial / physiology Liposomes Models, Molecular Nitrate Transporters Nitrates / metabolism* Nitrites / metabolism* Protein Binding Protein Conformation |
| IF | 12.121 |
| 引用数 | 19 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | Genomic DNA of Escherichia coli JCM 20135 (JGD07547) |
| 一般微生物 | |